General Noradrenaline (NE) ELISA Kit | NE elisa kit

General Noradrenaline (NE) ELISA Kit

Cat. Num. AAA2000232

• Reactivity: General
• Synonyms: Noradrenaline (NE); General Noradrenaline (NE) ELISA Kit; NA; NAd; Norepinephrine; Norepi; NE elisa kit

• Reactivity: General
• Specificity: This assay has high sensitivity and excellent specificity for detection of Noradrenaline (NE).; No significant cross-reactivity or interference between Noradrenaline (NE) and analogues was observed.
• Sequence Length: 617

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 61.7-5000pg/mL
• Sensitivity: < 26.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Noradrenaline (NE) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Noradrenaline (NE) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NE elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Noradrenaline (NE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Noradrenaline (NE) and unlabeled Noradrenaline (NE) (Standards or samples) with the pre-coated antibody specific to Noradrenaline (NE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Noradrenaline (NE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Noradrenaline (NE) in the sample.

Product Categories/Family for NE elisa kit

Signal transduction; Infection immunity; Neuroscience; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 189258
• NCBI GeneID: 6530
• UniProt Accession #: P23975; Q96KH8; B2R707; B4DX48
• Molecular Weight: 57,370 Da
• NCBI Official Full Name: noradrenaline transporter
• UniProt Protein Name: Sodium-dependent noradrenaline transporter
• UniProt Gene Name: SLC6A2
• UniProt Synonym Gene Names: NAT1; NET1; SLC6A5; NET
• UniProt Entry Name: SC6A2_HUMAN

NCBI Description

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Uniprot Description

SLC6A2: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A2 are a cause of orthostatic intolerance (OI). OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: neuron projection; cell surface; membrane; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; monoamine transmembrane transporter activity; beta-tubulin binding; alpha-tubulin binding; norephinephrine:sodium symporter activity

Biological Process: response to drug; synaptic transmission; norepinephrine transport; neurotransmitter transport; transport; monoamine transport; response to pain; transmembrane transport

Disease: Orthostatic Intolerance