Mouse Myogenin (MYOG) RTU ELISA Kit | MYOG rtu elisa kit

Mouse Myogenin (MYOG) ELISA Kit

Cat. Num. AAA4501267

• Gene Names: Myog; myo; MYF4; bHLHc3
• Reactivity: Mouse
• Synonyms: Myogenin (MYOG); Mouse Myogenin (MYOG) ELISA Kit; MYF4; bHLHc3; Myogenic Factor 4; Class C basic helix-loop-helix protein 3; MYOG rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 224

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.108ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYOG rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYOG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MYOG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYOG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MYOG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13654247
• NCBI GeneID: 17928
• NCBI Accession #: NP_112466.1
• NCBI GenBank Nucleotide #: NM_031189.2
• UniProt Accession #: P12979
• Molecular Weight: 25,203 Da
• NCBI Official Full Name: myogenin
• NCBI Official Synonym Full Names: myogenin
• NCBI Official Symbol: Myog
• NCBI Official Synonym Symbols: myo; MYF4; bHLHc3
• NCBI Protein Information: myogenin
• UniProt Protein Name: Myogenin
• Protein Family: Myosin
• UniProt Gene Name: Myog

Uniprot Description

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.