Rabbit anti-Human, Mouse MUTYH Polyclonal Antibody | anti-MUTYH antibody

MUTYH, ID (MUTYH, MYH, A/G-specific adenine DNA glycosylase, MutY homolog) (MaxLight 650)

Cat. Num. AAA6323063

• Gene Names: MUTYH; MYH; CYP2C
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: MUTYH; Polyclonal Antibody; ID (MUTYH; MYH; A/G-specific adenine DNA glycosylase; MutY homolog) (MaxLight 650); MutY homolog; anti-MUTYH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MUTYH. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-MUTYH antibody: Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 341-367 in the central region of human MUTYH.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS645261 in mouse lung tissue lysates (35ug/lane). MUTYH (arrow) was detected usingMBS645261.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Hela cells using MBS645261 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H460 cells using MBS645261 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MUTYH antibody

MYH is the eukaryotic homolog to the E. coli protein MutY. It was first identified in HeLa cells and nicks DNA replication errors, specifically A/G mismatches. MYH is involved in oxidative DNA damage repair. It initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. It possesses both adenine and 2-OH-A DNA glycosylase activities.

Product Categories/Family for anti-MUTYH antibody

Antibodies; Enzymes

References

1. Kim C.J., et.al., Oncogene 23:6820-6822(2004). 2. Isidro G., et.al., Hum. Mutat. 24:353-354(2004). 3. Sieber O.M., et.al., N. Engl. J. Med. 348:791-799(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 115298648
• NCBI GeneID: 4595
• NCBI Accession #: NP_001041636.1
• NCBI GenBank Nucleotide #: NM_001048171.1
• UniProt Accession #: Q9UIF7; Q15830; Q9UBP2; Q9UBS7; Q9UIF4; Q9UIF5; Q9UIF6; D3DPZ4
• Molecular Weight: 60,069 Da
• NCBI Official Full Name: A/G-specific adenine DNA glycosylase isoform 2
• NCBI Official Synonym Full Names: mutY homolog (E. coli)
• NCBI Official Symbol: MUTYH
• NCBI Official Synonym Symbols: MYH; CYP2C
• NCBI Protein Information: A/G-specific adenine DNA glycosylase
• UniProt Protein Name: A/G-specific adenine DNA glycosylase
• UniProt Gene Name: MUTYH
• UniProt Synonym Gene Names: MYH; hMYH
• UniProt Entry Name: MUTYH_HUMAN

NCBI Description

This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MUTYH: Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2- OH-A DNA glycosylase activities. Belongs to the Nth/MutY family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 3.2.2.-; Tumor suppressor; Hydrolase; Mitochondrial

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; 4 iron, 4 sulfur cluster binding; metal ion binding; hydrolase activity, acting on glycosyl bonds; MutLalpha complex binding; MutSbeta complex binding; MutLbeta complex binding; MutSalpha complex binding

Biological Process: mismatch repair; depurination; base-excision repair, AP site formation; base-excision repair; DNA repair

Disease: Gastric Cancer; Pilomatrixoma; Familial Adenomatous Polyposis, 2