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Western Blot (WB) (Western Blot: Sample: Rat Liver lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat GSTt1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Rat Glutathione S Transferase Theta 1 Polyclonal Antibody | anti-GSTt1 antibody

Polyclonal Antibody to Glutathione S Transferase Theta 1 (GSTt1)

Gene Names
Gstt1; GSTYRS
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Glutathione S Transferase Theta 1; Polyclonal Antibody; Polyclonal Antibody to Glutathione S Transferase Theta 1 (GSTt1); anti-GSTt1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GSTt1. It has beenselected for its ability to recognize GSTt1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LDLL SQPCRAIYIF AKKNNIPFQM HTVELRKGEH LSDAFAQVNP MKKVPAMKDG GFTLCESVAI LLYLAHKYKV PDHWYPQDLQ ARARVDEYLA WQHTTLRRSC LRTLWHKVMF PVFLGEQIRP EMLAATLADL DVNVQVLEDQ FLQDKDFLVG PHISLADVVA ITELMHPVGG GCPVFEGRPR LAAWYRRVEA AVGKDLFLEA HEVILKVRDC PPADPVIKQK L
Sequence Length
240
Applicable Applications for anti-GSTt1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
GSTt1 (Leu7~Leu231)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant GSTt1 (Leu7~Leu231) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2045119
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Liver lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat GSTt1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Liver lysate; Primary Ab: 3ug/ml Rabbit Anti-Rat GSTt1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded skin tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded skin tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,468 Da
NCBI Official Full Name
glutathione S-transferase theta-1
NCBI Official Synonym Full Names
glutathione S-transferase theta 1
NCBI Official Symbol
Gstt1
NCBI Official Synonym Symbols
GSTYRS
NCBI Protein Information
glutathione S-transferase theta-1
UniProt Protein Name
Glutathione S-transferase theta-1
Protein Family
UniProt Gene Name
Gstt1

NCBI Description

subunit of the glutathione S-transferase [RGD, Feb 2006]

Uniprot Description

GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family.

Protein type: EC 2.5.1.18; Other Amino Acids Metabolism - glutathione; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: cytosol; nucleus

Molecular Function: alkylhalidase activity; glutathione peroxidase activity; glutathione transferase activity

Biological Process: dichloromethane metabolic process; DNA modification; glutathione metabolic process; response to drug; response to organic cyclic compound; response to salicylic acid; response to selenium ion; response to vitamin E

Research Articles on GSTt1

Similar Products

Product Notes

The GSTt1 gstt1 (Catalog #AAA2027416) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutathione S Transferase Theta 1 (GSTt1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione S Transferase Theta 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the GSTt1 gstt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LDLL SQPCRAIYIF AKKNNIPFQM HTVELRKGEH LSDAFAQVNP MKKVPAMKDG GFTLCESVAI LLYLAHKYKV PDHWYPQDLQ ARARVDEYLA WQHTTLRRSC LRTLWHKVMF PVFLGEQIRP EMLAATLADL DVNVQVLEDQ FLQDKDFLVG PHISLADVVA ITELMHPVGG GCPVFEGRPR LAAWYRRVEA AVGKDLFLEA HEVILKVRDC PPADPVIKQK L. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase Theta 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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