Western Blot (WB)
(Western blotting analysis of human TROP2 using mouse monoclonal antibody TrMab-6 on lysates of MCF-7, SK-BR-3, and HaCaT cell lines, TROP2-positive and TROP2-negative DU-145 cells, wild-type T-47D and TROP2 knock-out T-47D cells, and TROP2 over-expressing and knock-out MDA-MB-231 cells. Primary antibody concentration was 1ug/ml. Tested by Ond?ej Vacek and Karel Sou?ek, Institute of Biophysics of the Czech Academy of Sciences.)
Mouse anti-Pig, Human TROP2 Antibody | anti-TROP2 antibody
Anti-Hu TROP2 Purified
WB: Recommended dilution: 1ug/ml.
ICC: Recommended dilution: 10ug/ml; the cells can be fixed with 4% PFA and permeabilized with 0.1% Triton X-100 before antibody staining, when needed.
IHC-P: Recommended dilution: 10ug/ml. Heat-mediated antigen retrieval in citrate buffer, pH= 6.
Western Blot (WB)
(Western blotting analysis of human TROP2 using mouse monoclonal antibody TrMab-6 on lysates of MCF-7, SK-BR-3, and HaCaT cell lines, TROP2-positive and TROP2-negative DU-145 cells, wild-type T-47D and TROP2 knock-out T-47D cells, and TROP2 over-expressing and knock-out MDA-MB-231 cells. Primary antibody concentration was 1ug/ml. Tested by Ond?ej Vacek and Karel Sou?ek, Institute of Biophysics of the Czech Academy of Sciences.)
Western Blot (WB)
(Western blotting analysis of human TROP2 using mouse monoclonal antibody TrMab-6 on lysates of MCF-7, SK-BR-3, and HaCaT cell lines, TROP2-positive and TROP2-negative DU-145 cells, wild-type T-47D and TROP2 knock-out T-47D cells, and TROP2 over-expressing and knock-out MDA-MB-231 cells. Primary antibody concentration was 1ug/ml. Tested by Ond?ej Vacek and Karel Sou?ek, Institute of Biophysics of the Czech Academy of Sciences.)
Flow Cytometry (FC/FACS)
(Separation of A431 cells (red-filled) from human peripheral whole blood cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human TROP-2 (TrMab-6) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)
Flow Cytometry (FC/FACS)
(Separation of A431 cells (red-filled) from human peripheral whole blood cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human TROP-2 (TrMab-6) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of TROP2 on the surface of unfixed wild-type and TROP2-overexpressing MDA-MB-231 cells using mouse monoclonal antibody TrMab-6 (1 ug/ml), detected with GAM IgG-Alexa Fluor488 (diluted 1:1000, green); isotype control IgG2b was used as the negative control. DNA stained with DAPI (blue). Tested by Ond?ej Vacek and Karel Sou?ek, Institute of Biophysics of the Czech Academy of Sciences.)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of TROP2 on the surface of unfixed wild-type and TROP2-overexpressing MDA-MB-231 cells using mouse monoclonal antibody TrMab-6 (1 ug/ml), detected with GAM IgG-Alexa Fluor488 (diluted 1:1000, green); isotype control IgG2b was used as the negative control. DNA stained with DAPI (blue). Tested by Ond?ej Vacek and Karel Sou?ek, Institute of Biophysics of the Czech Academy of Sciences.)
Immunohistochemistry (IHC)
(Immunohistochemistry staining (paraffin sections) of TROP2 in human and porcine lung tissue using mouse monoclonal antibody TrMab-6 (diluted 1:100), detected with En Vision FLEX/HRP. Positive in basolateral parts of airway epithelium. Negative control was stained without primary antibody- Scale bar represents 100 um. Tested by Vendula Pelková and Aleš Hampl, Department of Histology and Embryology at Faculty of Medicine, Masaryk University, Czech Republic.)
Immunohistochemistry (IHC)
(Immunohistochemistry staining (paraffin sections) of TROP2 in human and porcine lung tissue using mouse monoclonal antibody TrMab-6 (diluted 1:100), detected with En Vision FLEX/HRP. Positive in basolateral parts of airway epithelium. Negative control was stained without primary antibody- Scale bar represents 100 um. Tested by Vendula Pelková and Aleš Hampl, Department of Histology and Embryology at Faculty of Medicine, Masaryk University, Czech Republic.)
NCBI and Uniprot Product Information
NCBI Description
This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.[provided by RefSeq, Dec 2009]
Uniprot Description
TACSTD2: May function as a growth factor receptor. Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD); also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment. Belongs to the EPCAM family.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1p32
Cellular Component: extracellular space; membrane; integral to plasma membrane; basal plasma membrane; nucleus; cytosol; lateral plasma membrane
Molecular Function: protein binding; receptor activity
Biological Process: cell proliferation; cell surface receptor linked signal transduction; negative regulation of stress fiber formation; visual perception; regulation of epithelial cell proliferation
Disease: Corneal Dystrophy, Gelatinous Drop-like
Research Articles on TROP2
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Product Notes
The TROP2 tacstd2 (Catalog #AAA1802253) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu TROP2 Purified reacts with Pig, Human and may cross-react with other species as described in the data sheet. AAA Biotech's TROP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS)(QC tested), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P). FC/FACS: Recommended dilution: 1-4ug/ml. WB: Recommended dilution: 1ug/ml. ICC: Recommended dilution: 10ug/ml; the cells can be fixed with 4% PFA and permeabilized with 0.1% Triton X-100 before antibody staining, when needed. IHC-P: Recommended dilution: 10ug/ml. Heat-mediated antigen retrieval in citrate buffer, pH= 6. Researchers should empirically determine the suitability of the TROP2 tacstd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TROP2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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