Western Blot (WB)
(Western Blot: Samples. Lane1: Porcine Brain Tissue; Lane2: Porcine Heart Tissue;)
Mouse anti-Human Aspartate Aminotransferase (AST) Monoclonal Antibody | anti-AST antibody
Monoclonal Antibody to Aspartate Aminotransferase (AST)
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Samples. Lane1: Porcine Brain Tissue; Lane2: Porcine Heart Tissue;)
Western Blot (WB)
(Western Blot: Samples. Lane1: Porcine Brain Tissue; Lane2: Porcine Heart Tissue;)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue))
NCBI and Uniprot Product Information
NCBI Description
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
Uniprot Description
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.3; Transferase
Chromosomal Location of Human Ortholog: 10q24.2
Cellular Component: axon terminus; cytoplasm; cytosol; extracellular exosome; lysosome; mitochondrion; nucleoplasm; nucleus
Molecular Function: carboxylic acid binding; L-aspartate:2-oxoglutarate aminotransferase activity; L-cysteine:2-oxoglutarate aminotransferase activity; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding
Biological Process: 2-oxoglutarate metabolic process; amino acid biosynthetic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; cellular response to insulin stimulus; fatty acid homeostasis; gluconeogenesis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glutamate metabolic process; glycerol biosynthetic process; Notch signaling pathway; oxaloacetate metabolic process; response to glucocorticoid stimulus
Disease: Aspartate Aminotransferase, Serum Level Of, Quantitative Trait Locus 1
Research Articles on AST
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Product Notes
The AST got1 (Catalog #AAA2085892) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Aspartate Aminotransferase (AST) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aspartate Aminotransferase (AST) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/ml Immunocytochemistry in formalin fixed cells: 5-30ug/ml Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml Immunohistochemistry in paraffin section: 5-30ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Researchers should empirically determine the suitability of the AST got1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aspartate Aminotransferase (AST), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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