Western Blot (WB)
(Western Blot (WB) analysis of Synaptotagmin (M303) polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Rabbit Synaptotagmin Polyclonal Antibody | anti-M303 antibody
Synaptotagmin (M303) Polyclonal Antibody
Gene Names
SYT1; P65; SYT; BAGOS; SVP65
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
Synaptotagmin; Polyclonal Antibody; Synaptotagmin (M303) Polyclonal Antibody; Synaptotagmin-1; Synaptotagmin 1; Synaptotagmin I; SytI; SYT1; SVP65; SYT; anti-M303 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Synaptotagmin (M303) polyclonal antibody detects endogenous levels of Synaptotagmin protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
422
Applicable Applications for anti-M303 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 280-330 of Human Synaptotagmin.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Synaptotagmin (M303) polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of Synaptotagmin (M303) polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Synaptotagmin (M303) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Synaptotagmin (M303) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)
Related Product Information for anti-M303 antibody
Synaptotagmins are a large gene family of synaptic vesicle type III integral membrane proteins that function as regulators of both exocytosis and endocytosis and are involved in neurotransmitter secretion from small secretory vesicles. Calcium binds to Synaptotagmin I which triggers neurotransmitter release at the synapse. Synaptotagmin II is phosphorylated by WNK1 in a process that regulates calcium-dependent interactions. Synaptotagmin III is involved in calcium-dependent exocytosis of secretory vesicles in endocrine cells and neurons. Synaptotagmin IV is expressed in neuronal tissues, and has the highest mRNA levels in the hippocampus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 64kDa
NCBI Official Full Name
synaptotagmin-1 isoform 1
NCBI Official Synonym Full Names
synaptotagmin 1
NCBI Official Symbol
SYT1
NCBI Official Synonym Symbols
P65; SYT; BAGOS; SVP65
NCBI Protein Information
synaptotagmin-1
UniProt Protein Name
Synaptotagmin-1
Protein Family
UniProt Gene Name
SYT1
UniProt Synonym Gene Names
SVP65; SYT; SytI
UniProt Entry Name
SYT1_HUMAN
NCBI Description
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]
Research Articles on M303
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Product Notes
The M303 syt1 (Catalog #AAA3000903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Synaptotagmin (M303) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Synaptotagmin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the M303 syt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synaptotagmin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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