Mouse anti-Human CD50 Antibody | anti-CD50 antibody

Anti-Hu CD50 Purified Low Endotoxin

Cat. Num. AAA1800967

• Gene Names: ICAM3; CD50; CDW50; ICAM-R
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: CD50; Anti-Hu CD50 Purified Low Endotoxin; intercellular adhesion molecule 3; ICAM-3; ICAM-R; anti-CD50 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: MEM-04
• Specificity: The antibody MEM-04 reacts with an extracellular epitope of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 547

• Applicable Applications for anti-CD50 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Functional
• Application Notes: FC/FACS: QC tested
• Immunogen: Leukocytes of a patient suffering from LGL-type leukemia.
• Preparation and Storage: Store at 2-8 degree C.

Immunohistochemistry (IHC)-Paraffin

(Immunohistochemistry staining of human tonsil (paraffin sections) using anti-CD50 (MEM-04).Commercially tested by LifeSpan BioSciences.)

Related Product Information for anti-CD50 antibody

CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 167466207
• NCBI GeneID: 3385
• NCBI Accession #: NP_002153.2
• NCBI GenBank Nucleotide #: NP_002153.2
• UniProt Accession #: P32942
• NCBI Official Full Name: intercellular adhesion molecule 3 isoform 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 3
• NCBI Official Symbol: ICAM3
• NCBI Official Synonym Symbols: CD50; CDW50; ICAM-R
• NCBI Protein Information: intercellular adhesion molecule 3
• UniProt Protein Name: Intercellular adhesion molecule 3
• UniProt Gene Name: ICAM3
• UniProt Synonym Gene Names: ICAM-3
• UniProt Entry Name: ICAM3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]

Uniprot Description

ICAM3: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: integrin binding; protein binding; receptor binding

Biological Process: cell-cell adhesion; extracellular matrix organization and biogenesis; regulation of immune response; cell adhesion