Rabbit anti-Human CD50 Monoclonal Antibody | anti-ICAM3 antibody

Recombinant Anti-CD50 Antibody, Rabbit Monoclonal

Cat. Num. AAA8102305

• Gene Names: ICAM3; CD50; CDW50; ICAM-R
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: CD50; Monoclonal Antibody; Recombinant Anti-CD50 Antibody; Rabbit Monoclonal; CD50/ICAM-3 Antibody; Rabbit MAb; intercellular adhesion molecule 3; Anti-CD50 Antibody; Anti-CDW50 Antibody; Anti-ICAM-3 Antibody; Anti-ICAM-R Antibody; anti-ICAM3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 002
• Specificity: Human CD50/ICAM-3.; No cross-reactivity in ELISA with Mouse ICAM1, Human ICAM, Human VCAM1, Mouse VCAM1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ICAM3 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-1:500
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human CD50/ICAM-3 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD50/ICAM-3 (rh CD50/ICAM-3).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human ICAM3 in human tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human ICAM3 in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-ICAM3 antibody

The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting of three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes and is thus an important molecule for the first immune response. ICAM-3 comprises five immunoglobulin-like domains and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule but also as a potent signaling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that the CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. Also, the interactions of ICAM-3 and LFA-1 facilitate HIV-1-induced virological synapse formation between T cells. ICAM-3 is associated with an increase in cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer marker.

References

Berney SM, et al. (1999) ICAM-3 (CD50) cross-linking augments signaling in CD3-activated peripheral human T lymphocytes. J Leukoc Biol. 65(6): 867-74.van Buul JD, et al. (2004) ICAM-3 activation modulates cell-cell contacts of human bone marrow endothelial cells. J Vasc Res. 41(1): 28-37.Sugino H. (2005) ICAM-3, a ligand for DC-SIGN, was duplicated from ICAM-1 in mammalian evolution, but was lost in the rodent genome. FEBS Lett. 579(13): 2901-6.Park JK, et al. (2010) ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB. Int J Oncol. 36(1): 181-92.

NCBI and Uniprot Product Information

• NCBI GI #: 167466207
• NCBI GeneID: 3385
• NCBI Accession #: NP_002153.2
• NCBI GenBank Nucleotide #: NM_002162.3
• UniProt Accession #: P32942; Q6PD68
• Molecular Weight: 59,541 Da
• NCBI Official Full Name: intercellular adhesion molecule 3
• NCBI Official Synonym Full Names: intercellular adhesion molecule 3
• NCBI Official Symbol: ICAM3
• NCBI Official Synonym Symbols: CD50; CDW50; ICAM-R
• NCBI Protein Information: intercellular adhesion molecule 3; ICAM-3; intercellular adhesion molecule-3
• UniProt Protein Name: Intercellular adhesion molecule 3
• Protein Family: Intercellular adhesion molecule
• UniProt Gene Name: ICAM3
• UniProt Synonym Gene Names: ICAM-3
• UniProt Entry Name: ICAM3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM3: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Cell adhesion; Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: integrin binding; protein binding; receptor binding

Biological Process: cell-cell adhesion; extracellular matrix organization and biogenesis; regulation of immune response; cell adhesion