Mouse anti-Human C3aR Antibody | anti-C3aR antibody

Anti-Hu C3aR Purified

Cat. Num. AAA1802249

• Gene Names: C3AR1; AZ3B; C3AR; HNFAG09
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Purified by protein-A affinity chromatography.
• Synonyms: C3aR; Anti-Hu C3aR Purified; AZ3B; HNFAG09; complement C3a receptor 1; anti-C3aR antibody

• Host: Mouse
• Reactivity: Human
• Isotype: Mouse IgG2b
• Clone Number: HC3aRZ8
• Specificity: The mouse monoclonal antibody HC3aRZ8 recognizes an extracellular epitope of C3aR, a transmembrane chemotactic receptor for C3a anaphylatoxin.
• Purity/Purification: Purified by protein-A affinity chromatography.
• Form/Format: Phosphate buffered saline (PBS), pH7.4, 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-C3aR antibody: Flow Cytometry (FC/FACS)(QC tested), Immunohistochemistry-Frozen (IHC-F)
• Application Notes: FC/FACS: Recommended dilution: 1-4ug/ml.
• Immunogen: Human C3aR transfectants
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C3aR (HC3aRZ8) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)

Flow Cytometry (FC/FACS)

(Separation of human neutrophil granulocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human C3aR (HC3aRZ8) purified antibody (concentration in sample 1,7ug/ml, GAM APC).)

Related Product Information for anti-C3aR antibody

C3aR is a 7TM transmembrane protein associated with G proteins, and serves as a receptor for C3a complement fragment. It is expressed mainly on neutrophils, mast cells, basophils, eosinophils, dendritic cells, monocytes, and macrophages. Upon detection of its ligand, the activated C3aR signaling cascade results in degranulation, superoxide production, and chemotaxis.

References

Abdelbaset-Ismail A, Borkowska-Rzeszotek S, Kubis E, Bujko K, Brze?niakiewicz-Janus K, Bolkun L, Kloczko J, Moniuszko M, Basak GW, Wiktor-Jedrzejczak W, Ratajczak MZ: Activation of the complement cascade enhances motility of leukemic cells by downregulating expression of HO-1. Leukemia 2017 Feb;31(2):446-458., URL: https://pubmed.ncbi.nlm.nih.gov/27451975/
Larsen LF, Juel-Berg N, Hansen A, Hansen KS, Mills EN, van Ree R, Rådinger M, Poulsen LK, Jensen BM: No difference in human mast cells derived from peanut allergic versus non-allergic subjects. Immun Inflamm Dis. 2018 Dec;6(4):416-427, URL: https://pubmed.ncbi.nlm.nih.gov/29992767/
Reis ES, Chen H, Sfyroera G, Monk PN, Köhl J, Ricklin D, Lambris JD: C5a receptor-dependent cell activation by physiological concentrations of desarginated C5a: insights from a novel label-free cellular assay. J Immunol. 2012 Nov 15;189(10):4797-805., URL: https://pubmed.ncbi.nlm.nih.gov/23041570/,*Soruri A, Kiafard Z, Dettmer C, Riggert J, Köhl J, Zwirner J: IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo. J Immunol. 2003 Mar 15;170(6):3306-14., URL: https://pubmed.ncbi.nlm.nih.gov/12626590/
Kiafard Z, Tschernig T, Schweyer S, Bley A, Neumann D, Zwirner J: Use of monoclonal antibodies to assess expression of anaphylatoxin receptors in tubular epithelial cells of human, murine and rat kidneys. Immunobiology 2007;212(2):129-39., URL: https://pubmed.ncbi.nlm.nih.gov/17336833/

NCBI and Uniprot Product Information

• NCBI GI #: 30385606
• NCBI GeneID: 719
• UniProt Accession #: Q16581; O43771; Q92868
• Molecular Weight: 53,864 Da
• NCBI Official Full Name: complement component 3a receptor 1
• NCBI Official Synonym Full Names: complement component 3a receptor 1
• NCBI Official Symbol: C3AR1
• NCBI Official Synonym Symbols: AZ3B; C3AR; HNFAG09
• NCBI Protein Information: C3a anaphylatoxin chemotactic receptor; C3a-R; complement component 3 receptor 1
• UniProt Protein Name: C3a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C3AR1
• UniProt Synonym Gene Names: AZ3B; C3R1; HNFAG09; C3AR; C3a-R
• UniProt Entry Name: C3AR_HUMAN

Uniprot Description

C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; C3a anaphylatoxin receptor activity; complement component C3a receptor activity; phosphoinositide phospholipase C activity

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; blood circulation; metabolic process; complement receptor mediated signaling pathway; inflammatory response; chemotaxis