Sheep Complement Component 3a ELISA Kit | C3a elisa kit

Sheep Complement Component 3a ELISA Kit

Cat. Num. AAA764484

• Gene Names: C3AR1; AZ3B; C3AR; HNFAG09
• Reactivity: Sheep
• Synonyms: Complement Component 3a; Sheep Complement Component 3a ELISA Kit; C3a elisa kit

• Reactivity: Sheep
• Sequence Length: 482

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 5.625ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for C3a elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Sheep C3a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Sheep C3a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Sheep C3a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Sheep C3a can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 30385606
• NCBI GeneID: 719
• Molecular Weight: 53,864 Da
• NCBI Official Full Name: complement component 3a receptor 1
• NCBI Official Synonym Full Names: complement component 3a receptor 1
• NCBI Official Symbol: C3AR1
• NCBI Official Synonym Symbols: AZ3B; C3AR; HNFAG09
• NCBI Protein Information: C3a anaphylatoxin chemotactic receptor
• UniProt Protein Name: C3a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C3AR1
• UniProt Synonym Gene Names: AZ3B; C3R1; HNFAG09; C3AR; C3a-R
• UniProt Entry Name: C3AR_HUMAN

Uniprot Description

C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytosol; integral to plasma membrane; plasma membrane

Molecular Function: C3a anaphylatoxin receptor activity; complement component C3a binding; complement component C3a receptor activity; G-protein coupled receptor activity; phosphoinositide phospholipase C activity

Biological Process: blood circulation; chemotaxis; complement receptor mediated signaling pathway; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; inflammatory response; positive regulation of angiogenesis; positive regulation of smooth muscle cell proliferation; regulation of blood pressure; tolerance induction to nonself antigen