Rat Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) ELISA Kit | MYH6 elisa kit

Rat Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) ELISA Kit

Cat. Num. AAA2705105

• Gene Names: MYH6; ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
• Reactivity: Rat
• Synonyms: Myosin Heavy Chain 6; Cardiac Muscle; Alpha (MYH6); Rat Myosin Heavy Chain 6; Alpha (MYH6) ELISA Kit; ASD3; MYHC; MYHCA; MyHC-alpha; Cardiomyopathy; Hypertrophic 1; Myosin heavy chain; cardiac muscle alpha isoform; MYH6 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6).; No significant cross-reactivity or interference between Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) and analogues was observed.
• Sequence Length: 1939

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYH6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 156104908
• NCBI GeneID: 4624
• NCBI Accession #: NP_002462.2
• NCBI GenBank Nucleotide #: NM_002471.3
• UniProt Accession #: P13533; Q13943; Q14906; Q14907; A2RTX1; D9YZU2
• Molecular Weight: 223,735 Da
• NCBI Official Full Name: myosin-6
• NCBI Official Synonym Full Names: myosin heavy chain 6
• NCBI Official Symbol: MYH6
• NCBI Official Synonym Symbols: ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
• NCBI Protein Information: myosin-6
• UniProt Protein Name: Myosin-6
• Protein Family: Myosin
• UniProt Gene Name: MYH6
• UniProt Synonym Gene Names: MYHCA; MyHC-alpha

NCBI Description

Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Feb 2017]

Uniprot Description

MYH6: Muscle contraction. Defects in MYH6 are the cause of atrial septal defect type 3 (ASD3). ASD3 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Defects in MYH6 are the cause of familial hypertrophic cardiomyopathy type 14 (CMH14). It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations,and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH6 are the cause of cardiomyopathy dilated type 1EE (CMD1EE). It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH6 are the cause of susceptibility to sick sinus syndrome type 3 (SSS3). The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors. MYH6 variations are associated with susceptibility to sick sinus syndrome (PubMed:21378987). The lifetime risk of being diagnosed with sick sinus syndrome is higher for carriers of variant p.Arg721Trp than for non-carriers (PubMed:21378987).

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: cytosol; muscle myosin complex; myofibril; myosin complex; sarcomere

Molecular Function: actin binding; actin-dependent ATPase activity; ATP binding; ATPase activity; calmodulin binding; microfilament motor activity; myosin phosphatase activity; protein kinase binding

Biological Process: adult heart development; ATP metabolic process; atrial cardiac muscle morphogenesis; cardiac muscle contraction; cardiac muscle fiber development; in utero embryonic development; muscle contraction; muscle filament sliding; myofibril assembly; protein amino acid dephosphorylation; regulation of ATPase activity; regulation of blood pressure; regulation of heart contraction; regulation of heart rate; regulation of the force of heart contraction; sarcomere organization; striated muscle contraction; ventricular cardiac muscle tissue morphogenesis; visceral muscle development

Disease: Atrial Septal Defect 3; Cardiomyopathy, Dilated, 1ee; Cardiomyopathy, Familial Hypertrophic, 1; Cardiomyopathy, Familial Hypertrophic, 14; Sick Sinus Syndrome 3, Susceptibility To