Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Troponin T, slow skeletal muscle (TNNT1) Standard Curve.)
Rat Troponin T, Slow Skeletal Muscle (TNNT1) ELISA Kit | TNNT1 elisa kit
Rat Troponin T, Slow Skeletal Muscle (TNNT1) ELISA Kit
Gene Names
Tnnt1; sTnT; tnTs; Fang2
Reactivity
Rat
Synonyms
Troponin T; Slow Skeletal Muscle (TNNT1); Rat Troponin T; Slow Skeletal Muscle (TNNT1) ELISA Kit; TNNT1; ANM; FLJ98147; MGC104241; STNT; TNT; TNTS; slow skeletal muscle troponin T; troponin T1; skeletal; slow; troponin-T1; TNNT1 elisa kit
Reactivity
Rat
Specificity
Rat Troponin T, slow skeletal muscle (TNNT1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNNT1. No significant cross-reactivity or interference between Rat TNNT1 and analogues was observed.
Sequence Length
260
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Troponin T, slow skeletal muscle (TNNT1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Troponin T, slow skeletal muscle (TNNT1) Standard Curve.)
Related Product Information for TNNT1 elisa kit
Description: This Rat Troponin T, slow skeletal muscle (TNNT1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNT1 in samples. An antibody specific for TNNT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNNT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TNNT1 is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.
Background: TNNT1 is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
troponin T, slow skeletal muscle isoform 2
NCBI Official Synonym Full Names
troponin T1, slow skeletal type
NCBI Official Symbol
Tnnt1
NCBI Official Synonym Symbols
sTnT; tnTs; Fang2
NCBI Protein Information
troponin T, slow skeletal muscle
UniProt Protein Name
Troponin T, slow skeletal muscle
Protein Family
UniProt Gene Name
Tnnt1
UniProt Synonym Gene Names
Fang2; TnTs; sTnT
NCBI Description
human homolog plays a role in the calcium sensitivity of contraction in skeletal muscle [RGD, Feb 2006]
Uniprot Description
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
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Product Notes
The Rat TNNT1 tnnt1 (Catalog #AAA9711660) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711660 ELISA Kit recognizes Rat TNNT1. It is sometimes possible for the material contained within the vial of "Troponin T, Slow Skeletal Muscle (TNNT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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