Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Troponin I, slow skeletal muscle (TNNI1) Standard Curve.)
Mouse Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit | TNNI1 elisa kit
Mouse Troponin I, Slow Skeletal Muscle (TNNI1) ELISA Kit
Gene Names
Tnni1; ssTnI; AI747285; 2700018B22Rik
Reactivity
Mouse
Synonyms
Troponin I; Slow Skeletal Muscle (TNNI1); Mouse Troponin I; Slow Skeletal Muscle (TNNI1) ELISA Kit; TNNI1; DKFZp451O223; SSTNI; TNN1; OTTHUMP00000033877; OTTHUMP00000033910; slow-twitch skeletal troponin I; troponin I; skeletal; slow; TNNI1 elisa kit
Reactivity
Mouse
Specificity
Mouse Troponin I, slow skeletal muscle (TNNI1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNNI1. No significant cross-reactivity or interference between Mouse TNNI1 and analogues was observed.
Sequence Length
187
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Troponin I, slow skeletal muscle (TNNI1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Troponin I, slow skeletal muscle (TNNI1) Standard Curve.)
Related Product Information for TNNI1 elisa kit
Description: This Mouse Troponin I, slow skeletal muscle (TNNI1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNI1 in samples. An antibody specific for TNNI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNNI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. TNNI1 is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
Background: TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. TNNI1 is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
troponin I, slow skeletal muscle
NCBI Official Synonym Full Names
troponin I, skeletal, slow 1
NCBI Official Symbol
Tnni1
NCBI Official Synonym Symbols
ssTnI; AI747285; 2700018B22Rik
NCBI Protein Information
troponin I, slow skeletal muscle
UniProt Protein Name
Troponin I, slow skeletal muscle
Protein Family
UniProt Gene Name
Tnni1
UniProt Entry Name
TNNI1_MOUSE
Uniprot Description
TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Cellular Component: troponin complex
Molecular Function: actin binding; metal ion binding
Biological Process: cardiac muscle contraction; regulation of muscle contraction; skeletal muscle contraction; transition between fast and slow fiber; ventricular cardiac muscle morphogenesis
Research Articles on TNNI1
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Product Notes
The Mouse TNNI1 tnni1 (Catalog #AAA9716711) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716711 ELISA Kit recognizes Mouse TNNI1. It is sometimes possible for the material contained within the vial of "Troponin I, Slow Skeletal Muscle (TNNI1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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