Western Blot (WB)
(Anti-ESAM rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDaObserved band size:37 kDa)
Rabbit anti-Human ESAM Polyclonal Antibody | anti-ESAM antibody
Anti-ESAM Antibody, Rabbit Polyclonal
Gene Names
ESAM; W117m
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
ESAM; Polyclonal Antibody; Anti-ESAM Antibody; Rabbit Polyclonal; ESAM/Endothelial Cell Adhesion Molecule Antibody; Rabbit PAb; Antigen Affinity Purified; endothelial cell adhesion molecule; Anti-W117m Antibody; anti-ESAM antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ESAM
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ESAM antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant Human ESAM protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human ESAM extracellular domain (rh ESAM). ESAM specific IgG was purified by human ESAM affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ESAM rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDaObserved band size:37 kDa)
Western Blot (WB)
(Anti-ESAM rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDaObserved band size:37 kDa)
Related Product Information for anti-ESAM antibody
Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. It is specifically expressed at endothelial tight junctions and on activated platelets. ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. Previous studies have indicated that ESAM regulates angiogenesis in the primary tumor growth and endothelial permeability. It suggest that ESAM has a redundant functional role in physiological angiogenesis but serves a unique and essential role in pathological angiogenic processes such as tumor growth.
References
Ishida T, et al. (2003) Targeted disruption of endothelial cell-selective adhesion molecule inhibits angiogenic processes in vitro and in vivo. J Biol Chem. 278(36): 34598-604.Wegmann F, et al. (2004) Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts. Exp Cell Res. 300(1): 121-33.Wegmann F, et al. (2006) ESAM supports neutrophil extravasation, activation of Rho, and VEGF-induced vascular permeability. J Exp Med. 203(7): 1671-7.Hara T, et al. (2009) Endothelial cell-selective adhesion molecule regulates albuminuria in diabetic nephropathy. Microvasc Res. 77(3): 348-55.Cangara HM, et al. (2010) Role of endothelial cell-selective adhesion molecule in hematogeneous metastasis. Microvasc Res. 80(1): 133-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,170 Da
NCBI Official Full Name
endothelial cell-selective adhesion molecule
NCBI Official Synonym Full Names
endothelial cell adhesion molecule
NCBI Official Symbol
ESAM
NCBI Official Synonym Symbols
W117m
NCBI Protein Information
endothelial cell-selective adhesion molecule
UniProt Protein Name
Endothelial cell-selective adhesion molecule
Protein Family
UniProt Gene Name
ESAM
Uniprot Description
Can mediate aggregation most likely through a homophilic molecular interaction.
Research Articles on ESAM
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Product Notes
The ESAM esam (Catalog #AAA8101572) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ESAM Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ESAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the ESAM esam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ESAM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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