Mouse TFEB (Transcription factor EB) ELISA Kit | TFEB elisa kit

Mouse TFEB (Transcription factor EB) ELISA Kit

Cat. Num. AAA7612403

• Gene Names: Tfeb; Tcfeb; bHLHe35
• Reactivity: Mouse
• Synonyms: TFEB (Transcription factor EB); Mouse TFEB (Transcription factor EB) ELISA Kit; Transcription factor EB; Tcfeb; Tfeb; TFEB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TFEB. No significant cross-reactivity or interference between TFEB and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFEB elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 239835746
• NCBI GeneID: 21425
• NCBI Accession #: NP_001155194.1
• NCBI GenBank Nucleotide #: NM_001161722.1
• UniProt Accession #: Q9R210
• Molecular Weight: 52,614 Da
• NCBI Official Full Name: transcription factor EB isoform b
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: Tfeb
• NCBI Official Synonym Symbols: Tcfeb; bHLHe35
• NCBI Protein Information: transcription factor EB
• UniProt Protein Name: Transcription factor EB
• Protein Family: Transcription factor
• UniProt Gene Name: Tfeb
• UniProt Synonym Gene Names: Tcfeb
• UniProt Entry Name: TFEB_MOUSE

Uniprot Description

TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: protein dimerization activity; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; embryonic placenta development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; immune system process; lysosome organization and biogenesis; positive regulation of transcription, DNA-dependent; autophagy; positive regulation of transcription from RNA polymerase II promoter; humoral immune response; positive regulation of autophagy