Mouse REST (RE1-Silencing Transcription Factor) ELISA Kit | REST elisa kit

Mouse REST (RE1-Silencing Transcription Factor) ELISA Kit

Cat. Num. AAA7612402

• Gene Names: Rest; NRSF; REST4; AA407358; 2610008J04Rik
• Reactivity: Mouse
• Synonyms: REST (RE1-Silencing Transcription Factor); Mouse REST (RE1-Silencing Transcription Factor) ELISA Kit; RE1-silencing transcription factor; Neural-restrictive silencer factor; Nrsf; Rest; REST elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of REST. No significant cross-reactivity or interference between REST and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for REST elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 254675343
• NCBI GeneID: 19712
• NCBI Accession #: NP_035393.2
• NCBI GenBank Nucleotide #: NM_011263.2
• UniProt Accession #: Q8VIG1; Q155C6; Q3U0W0; Q4VAD6; Q9CW43; A0JNY8
• Molecular Weight: 37,475 Da
• NCBI Official Full Name: RE1-silencing transcription factor
• NCBI Official Synonym Full Names: RE1-silencing transcription factor
• NCBI Official Symbol: Rest
• NCBI Official Synonym Symbols: NRSF; REST4; AA407358; 2610008J04Rik
• NCBI Protein Information: RE1-silencing transcription factor
• UniProt Protein Name: RE1-silencing transcription factor
• Protein Family: RE1-silencing transcription factor
• UniProt Gene Name: Rest
• UniProt Synonym Gene Names: Nrsf
• UniProt Entry Name: REST_MOUSE

Uniprot Description

REST: a transcriptional repressor that binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells during embryonic development. Induced during ageing in human cortical and hippocampal neurons, where it represses genes that promote cell death and induces the expression of stress response genes. Protects neurons from amyloid beta- and oxidative stress-induced toxicity. The deletion of REST in the mouse brain induces age-related neurodegeneration. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. REST activates DYRK1A transcription via a neuron-restricted silencer element in the DYRK1A promoter. Four isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Cell development/differentiation; DNA-binding; C2H2-type zinc finger protein

Cellular Component: protein complex; transcriptional repressor complex; nucleus; chromatin; cytosol

Molecular Function: protein binding; nucleic acid binding; DNA binding; outward rectifier potassium channel activity; metal ion binding; protein complex binding; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; negative regulation of calcium ion-dependent exocytosis; transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of neurogenesis; negative regulation of insulin secretion; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; negative regulation of aldosterone biosynthetic process; hemopoietic progenitor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent