Human beta Arrestin-2 (Beta Arrestin-2) ELISA Kit | Arrb2 elisa kit

Rat beta Arrestin-2 (Beta Arrestin-2) ELISA Kit

Cat. Num. AAA7612434

• Gene Names: Arrb2; BARRES
• Reactivity: Human
• Synonyms: beta Arrestin-2 (Beta Arrestin-2); Rat beta Arrestin-2 (Beta Arrestin-2) ELISA Kit; Beta-arrestin-2; Arrestin beta-2; Arrb2; Arrb2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of beta Arrestin-2. No significant cross-reactivity or interference between beta Arrestin-2 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Arrb2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6978539
• NCBI GeneID: 25388
• NCBI Accession #: NP_037043.1
• NCBI GenBank Nucleotide #: NM_012911.1
• UniProt Accession #: P29067
• Molecular Weight: 46,340 Da
• NCBI Official Full Name: beta-arrestin-2
• NCBI Official Synonym Full Names: arrestin, beta 2
• NCBI Official Symbol: Arrb2
• NCBI Official Synonym Symbols: BARRES
• NCBI Protein Information: beta-arrestin-2
• UniProt Protein Name: Beta-arrestin-2
• Protein Family: Beta-arrestin
• UniProt Gene Name: Arrb2
• UniProt Entry Name: ARRB2_RAT

Uniprot Description

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes.

Protein type: Adaptor/scaffold

Cellular Component: basolateral plasma membrane; coated pit; cytoplasm; cytoplasmic vesicle; dendritic spine; endocytic vesicle; intracellular; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane

Molecular Function: alpha-1A adrenergic receptor binding; alpha-1B adrenergic receptor binding; angiotensin receptor binding; clathrin binding; D1 dopamine receptor binding; enzyme binding; follicle stimulating hormone receptor binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; mitogen-activated protein kinase binding; platelet activating factor receptor binding; protein binding; protein complex binding; protein complex scaffold; protein domain specific binding; protein kinase B binding; receptor binding; type 1 angiotensin receptor binding; type 2A serotonin receptor binding; ubiquitin protein ligase binding

Biological Process: adult walking behavior; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; brain development; detection of temperature stimulus involved in sensory perception of pain; endocytosis; follicle-stimulating hormone signaling pathway; G-protein coupled receptor internalization; G-protein coupled receptor protein signaling pathway; inhibition of NF-kappaB transcription factor; negative regulation of caspase activity; negative regulation of interleukin-1 beta production; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of protein ubiquitination; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; positive regulation of calcium ion transport; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of protein ubiquitination; positive regulation of receptor internalization; positive regulation of synaptic transmission, dopaminergic; proteasomal ubiquitin-dependent protein catabolic process; protein transport; protein ubiquitination; receptor internalization; regulation of cAMP biosynthetic process; regulation of cAMP catabolic process; regulation of G-protein coupled receptor protein signaling pathway; regulation of protein amino acid phosphorylation; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway