Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Semaphorin-3F (SEMA3F) Standard Curve.)
Mouse Semaphorin-3F (SEMA3F) ELISA Kit | SEMA3F elisa kit
Mouse Semaphorin-3F (SEMA3F) ELISA Kit
Gene Names
Sema3f; Sema4; Semak
Reactivity
Mouse
Synonyms
Semaphorin-3F (SEMA3F); Mouse Semaphorin-3F (SEMA3F) ELISA Kit; SEMA3F; SEMA-IV; SEMA4; SEMAK; sema IV; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; 3F; semaphorin 3F; semaphorinI/F; semaphorin IV; SEMA3F elisa kit
Reactivity
Mouse
Specificity
Mouse Semaphorin-3F (SEMA3F) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SEMA3F. No significant cross-reactivity or interference between Mouse SEMA3F and analogues was observed.
Sequence Length
785
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Semaphorin-3F (SEMA3F) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Semaphorin-3F (SEMA3F) Standard Curve.)
Related Product Information for SEMA3F elisa kit
Description: This Mouse Semaphorin-3F (SEMA3F) ELISA Kit employs a two-site sandwich ELISA to quantitate SEMA3F in samples. An antibody specific for SEMA3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3F bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The semaphorins are a family of proteins that are involved in signaling. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62), a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.
Background: The semaphorins are a family of proteins that are involved in signaling. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62), a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
semaphorin-3F isoform 1
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
NCBI Official Symbol
Sema3f
NCBI Official Synonym Symbols
Sema4; Semak
NCBI Protein Information
semaphorin-3F
UniProt Protein Name
Semaphorin-3F
Protein Family
UniProt Gene Name
Sema3f
UniProt Synonym Gene Names
Sema IV
UniProt Entry Name
SEM3F_MOUSE
Uniprot Description
SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Cellular Component: extracellular region; extracellular space
Molecular Function: chemorepellent activity; semaphorin receptor binding
Biological Process: axon extension involved in axon guidance; axon guidance; branchiomotor neuron axon guidance; facial nerve structural organization; negative chemotaxis; negative regulation of axon extension involved in axon guidance; nerve development; neural crest cell migration; positive regulation of cell migration; trigeminal nerve structural organization
Research Articles on SEMA3F
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Product Notes
The Mouse SEMA3F sema3f (Catalog #AAA9717068) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717068 ELISA Kit recognizes Mouse SEMA3F. It is sometimes possible for the material contained within the vial of "Semaphorin-3F (SEMA3F), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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