Western Blot (WB)
(Western blot analysis of ALKBH5 expression in 293 cell line lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse ALKBH5 Polyclonal Antibody | anti-ALKBH5 antibody
ALKBH5 Antibody
Gene Names
ALKBH5; ABH5; OFOXD; OFOXD1
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ALKBH5; Polyclonal Antibody; ALKBH5 Antibody; ABH5; AlkB; alkylation repair homolog 5 (E. coli); alkylation repair homolog 5; Alkylated DNA repair protein alkB homolog 5; Alpha ketoglutarate dependent dioxygenase alkB homolog 5; OFOXD; OFOXD1; Oxoglutarate and iron-dependent oxygenase domain containing; Probable alpha ketoglutarate dependent dioxygenase ABH5; RNA demethylase ALKBH5; anti-ALKBH5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ALKBH5 antibody detects endogenous levels of total ALKBH5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
394
Applicable Applications for anti-ALKBH5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Nucleus Speckle
Tissue Specificity
Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (82%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of ALKBH5 expression in 293 cell line lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of ALKBH5 expression in 293 cell line lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-ALKBH5 antibody
Function: Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N6-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis (By similarity).
Subunit Structure: Monomer.
Similarity: Belongs to the alkB family.
Subunit Structure: Monomer.
Similarity: Belongs to the alkB family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 44 kDa
Predicted: 45 kDa
Predicted: 45 kDa
NCBI Official Full Name
RNA demethylase ALKBH5
NCBI Official Synonym Full Names
alkB homolog 5, RNA demethylase
NCBI Official Symbol
ALKBH5
NCBI Official Synonym Symbols
ABH5; OFOXD; OFOXD1
NCBI Protein Information
RNA demethylase ALKBH5
UniProt Protein Name
RNA demethylase ALKBH5
Protein Family
UniProt Gene Name
ALKBH5
UniProt Synonym Gene Names
ABH5; OFOXD1
Uniprot Description
Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N6-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for spermatogenesis ().
Research Articles on ALKBH5
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Product Notes
The ALKBH5 alkbh5 (Catalog #AAA9604099) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALKBH5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ALKBH5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ALKBH5 alkbh5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALKBH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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