Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Ribonuclease P protein subunit p20 (POP7) Standard Curve.)
Mouse Ribonuclease P Protein Subunit p20 (POP7) ELISA Kit | POP7 elisa kit
Mouse Ribonuclease P Protein Subunit p20 (POP7) ELISA Kit
Gene Names
Pop7; Rpp20; AI852017; 0610037N12Rik
Reactivity
Mouse
Synonyms
Ribonuclease P Protein Subunit p20 (POP7); Mouse Ribonuclease P Protein Subunit p20 (POP7) ELISA Kit; POP7; tcag7.985; 0610037N12Rik; RPP2; RPP20; POP7 (processing of precursor; S. cerevisiae) homolog; processing of precursor 7; ribonuclease P subunit; ribonuclease P protein subuni; POP7 elisa kit
Reactivity
Mouse
Specificity
Mouse Ribonuclease P protein subunit p20 (POP7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POP7. No significant cross-reactivity or interference between Mouse POP7 and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Ribonuclease P protein subunit p20 (POP7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Ribonuclease P protein subunit p20 (POP7) Standard Curve.)
Related Product Information for POP7 elisa kit
Description: This Mouse Ribonuclease P protein subunit p20 (POP7) ELISA Kit employs a two-site sandwich ELISA to quantitate POP7 in samples. An antibody specific for POP7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POP7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POP7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POP7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Ribonuclease P (RNase P) removes the 5-prime leader sequences from precursor tRNA molecules. RNase P consists of an RNA species (H1 RNA), the POP1 protein, and at least 7 proteins called RPPs. The RPPs have apparent molecular masses of 14 kD (RPP14), 20 kD (RPP20), 25 kD (RPP25), 29 kD (RPP29), 30 kD (RPP30), 38 kD (RPP38), and 40 kD (RPP40). Patients with scleroderma have serum reactive with RNase P, the Th antigen, which is also referred to as the To antigen, and RPP30 and RPP38. POP7 contains 140 amino acids with a predicted molecular mass of nearly 16 kD. Immunologic analysis determined that RPP20, unlike RPP30 and RPP38, is not a target for antisera from systemic sclerosis patients.
Background: Ribonuclease P (RNase P) removes the 5-prime leader sequences from precursor tRNA molecules. RNase P consists of an RNA species (H1 RNA), the POP1 protein, and at least 7 proteins called RPPs. The RPPs have apparent molecular masses of 14 kD (RPP14), 20 kD (RPP20), 25 kD (RPP25), 29 kD (RPP29), 30 kD (RPP30), 38 kD (RPP38), and 40 kD (RPP40). Patients with scleroderma have serum reactive with RNase P, the Th antigen, which is also referred to as the To antigen, and RPP30 and RPP38. POP7 contains 140 amino acids with a predicted molecular mass of nearly 16 kD. Immunologic analysis determined that RPP20, unlike RPP30 and RPP38, is not a target for antisera from systemic sclerosis patients.
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
NCBI Official Synonym Full Names
processing of precursor 7, ribonuclease P family, (S. cerevisiae)
NCBI Official Symbol
Pop7
NCBI Official Synonym Symbols
Rpp20; AI852017; 0610037N12Rik
NCBI Protein Information
ribonuclease P protein subunit p20
UniProt Protein Name
Ribonuclease P protein subunit p20
Protein Family
UniProt Gene Name
Pop7
UniProt Synonym Gene Names
Rpp20; RNaseP protein p20
UniProt Entry Name
POP7_MOUSE
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Product Notes
The Mouse POP7 pop7 (Catalog #AAA9718050) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718050 ELISA Kit recognizes Mouse POP7. It is sometimes possible for the material contained within the vial of "Ribonuclease P Protein Subunit p20 (POP7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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