Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ribonuclease pancreatic (RNASE1/RIB1/RNS1) Standard Curve.)
Human Ribonuclease Pancreatic (RNASE1/RIB1/RNS1) ELISA Kit | RNASE1/RIB1/RNS1 elisa kit
Human Ribonuclease Pancreatic (RNASE1/RIB1/RNS1) ELISA Kit
Gene Names
RNASE1; RAC1; RIB1; RNS1
Reactivity
Human
Synonyms
Ribonuclease Pancreatic (RNASE1/RIB1/RNS1); Human Ribonuclease Pancreatic (RNASE1/RIB1/RNS1) ELISA Kit; RNASE1; MGC12408; RIB1; RNS1; RNase upI-1; pancreatic ribonuclease; RNASE1/RIB1/RNS1 elisa kit
Reactivity
Human
Specificity
Human Ribonuclease pancreatic (RNASE1/RIB1/RNS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RNASE1. No significant cross-reactivity or interference between Human RNASE1 and analogues was observed.
Sequence Length
156
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ribonuclease pancreatic (RNASE1/RIB1/RNS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ribonuclease pancreatic (RNASE1/RIB1/RNS1) Standard Curve.)
Related Product Information for RNASE1/RIB1/RNS1 elisa kit
Description: This Human Ribonuclease pancreatic (RNASE1/RIB1/RNS1) ELISA Kit employs a two-site sandwich ELISA to quantitate RNASE1 in samples. An antibody specific for RNASE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RNASE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Pancreatic ribonucleases (EC 3.1.27.5, RNase, RNase I, RNase A, pancreatic RNase, ribonuclease I, endoribonuclease I, ribonucleic phosphatase, alkaline ribonuclease, ribonuclease, gene S glycoproteins, Ceratitis capitata alkaline ribonuclease, SLSG glycoproteins, gene S locus-specific glycoproteins, S-genotype-assocd. glycoproteins, ribonucleate 3'-pyrimidino-oligonucleotidohydrolase) are pyrimidine-specific endonucleases found in high quantity in the pancreas of certain mammals and of some reptiles. Specifically, the enzymes are involved in endonucleolytic cleavage of 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates. Ribonuclease can unwind the RNA helix by complexing with single-stranded RNA; the complex arises by an extended multi-site cation-anion interaction between lysine and arginine residues of the enzyme and phosphate groups of the nucleotides
Background: Pancreatic ribonucleases (EC 3.1.27.5, RNase, RNase I, RNase A, pancreatic RNase, ribonuclease I, endoribonuclease I, ribonucleic phosphatase, alkaline ribonuclease, ribonuclease, gene S glycoproteins, Ceratitis capitata alkaline ribonuclease, SLSG glycoproteins, gene S locus-specific glycoproteins, S-genotype-assocd. glycoproteins, ribonucleate 3'-pyrimidino-oligonucleotidohydrolase) are pyrimidine-specific endonucleases found in high quantity in the pancreas of certain mammals and of some reptiles. Specifically, the enzymes are involved in endonucleolytic cleavage of 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates. Ribonuclease can unwind the RNA helix by complexing with single-stranded RNA; the complex arises by an extended multi-site cation-anion interaction between lysine and arginine residues of the enzyme and phosphate groups of the nucleotides
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ribonuclease pancreatic
NCBI Official Synonym Full Names
ribonuclease A family member 1, pancreatic
NCBI Official Symbol
RNASE1
NCBI Official Synonym Symbols
RAC1; RIB1; RNS1
NCBI Protein Information
ribonuclease pancreatic
UniProt Protein Name
Ribonuclease pancreatic
UniProt Gene Name
RNASE1
UniProt Synonym Gene Names
RIB1; RNS1; RNase 1; RNase A
NCBI Description
This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single-stranded and double-stranded RNA.
Research Articles on RNASE1/RIB1/RNS1
1. Data suggest that ribonuclease inhibitor (RNH1) protects HeLa cells from ribonuclease 1 (RNase 1).
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Product Notes
The Human RNASE1/RIB1/RNS1 rnase1 (Catalog #AAA9714454) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714454 ELISA Kit recognizes Human RNASE1/RIB1/RNS1. It is sometimes possible for the material contained within the vial of "Ribonuclease Pancreatic (RNASE1/RIB1/RNS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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