Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prickle-like protein 3 (PRICKLE3) Standard Curve.)
Mouse Prickle-Like Protein 3 (PRICKLE3) ELISA Kit | PRICKLE3 elisa kit
Mouse Prickle-Like Protein 3 (PRICKLE3) ELISA Kit
Gene Names
Prickle3; Pk3; Lmo6; Sfc16
Reactivity
Mouse
Synonyms
Prickle-Like Protein 3 (PRICKLE3); Mouse Prickle-Like Protein 3 (PRICKLE3) ELISA Kit; PRICKLE3; LMO6; LIM domain only 6; PRICKLE3 elisa kit
Reactivity
Mouse
Specificity
Mouse Prickle-like protein 3 (PRICKLE3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRICKLE3. No significant cross-reactivity or interference between Mouse PRICKLE3 and analogues was observed.
Sequence Length
624
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prickle-like protein 3 (PRICKLE3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prickle-like protein 3 (PRICKLE3) Standard Curve.)
Related Product Information for PRICKLE3 elisa kit
Description: This Mouse Prickle-like protein 3 (PRICKLE3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRICKLE3 in samples. An antibody specific for PRICKLE3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRICKLE3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRICKLE3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRICKLE3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: In the study of a gene-rich region of the X chromosome, Xp11.23-p11.22, Fisher et al. (1997) identified a novel locus consisting of 8 exons in a 12.5-kb interval between the A4 and the SYP loci. This gene, called LMO6, was predicted to encode a product of 407 amino acids with significant homology to proteins containing LIM domains, in particular, mouse testin. The LIM domain is a cysteine-rich sequence motif that binds zinc atoms to form a specific protein-binding interface in protein-protein interactions. Like testin, the LMO6 product contains 3 such domains. Using an RT-PCR approach, they were able to amplify a 450-bp product spanning exons 5 to 7 of the LMO6 transcript from lymphoblastoid RNA. This confirmed that the LMO6 locus is indeed transcribed and unlikely to be a pseudogene.
Background: In the study of a gene-rich region of the X chromosome, Xp11.23-p11.22, Fisher et al. (1997) identified a novel locus consisting of 8 exons in a 12.5-kb interval between the A4 and the SYP loci. This gene, called LMO6, was predicted to encode a product of 407 amino acids with significant homology to proteins containing LIM domains, in particular, mouse testin. The LIM domain is a cysteine-rich sequence motif that binds zinc atoms to form a specific protein-binding interface in protein-protein interactions. Like testin, the LMO6 product contains 3 such domains. Using an RT-PCR approach, they were able to amplify a 450-bp product spanning exons 5 to 7 of the LMO6 transcript from lymphoblastoid RNA. This confirmed that the LMO6 locus is indeed transcribed and unlikely to be a pseudogene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prickle planar cell polarity protein 3
NCBI Official Synonym Full Names
prickle planar cell polarity protein 3
NCBI Official Symbol
Prickle3
NCBI Official Synonym Symbols
Pk3; Lmo6; Sfc16
NCBI Protein Information
prickle planar cell polarity protein 3; prickle-like protein 3
UniProt Protein Name
Prickle-like protein 3
Protein Family
UniProt Gene Name
Prickle3
UniProt Synonym Gene Names
Lmo6; LMO-6
UniProt Entry Name
PRIC3_MOUSE
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Product Notes
The Mouse PRICKLE3 prickle3 (Catalog #AAA9717286) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717286 ELISA Kit recognizes Mouse PRICKLE3. It is sometimes possible for the material contained within the vial of "Prickle-Like Protein 3 (PRICKLE3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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