CD97 recombinant protein

Recombinant Human CD97 Protein (His tag)

Cat. Num. AAA2545829

• Gene Names: ADGRE5; CD97; TM7LN1
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: CD97; Recombinant Human CD97 Protein (His tag); TM7LN1; CD97 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 742

• Application Notes: The recombinant human CD97 consists of 389 amino acids and predictes a molecular mass of 42.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD97 is approximately 60-70 kDa due to glycosylation.
• Predicted N Terminal: Gln 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD97 recombinant protein

A DNA sequence encoding the first 398 amino acids (Met 1-Gln 398) of human CD97 isoform 2 (NP_001775.2) extracellular domain was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 17978489
• NCBI GeneID: 976
• NCBI Accession #: NP_001775.2
• NCBI GenBank Nucleotide #: NM_001784.4
• UniProt Accession #: P48960; O00718; O76101; Q8NG72; Q8TBQ7; A8K7Z4; B2RBJ9
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: CD97 antigen isoform 2 preproprotein
• NCBI Official Synonym Full Names: adhesion G protein-coupled receptor E5
• NCBI Official Symbol: ADGRE5
• NCBI Official Synonym Symbols: CD97; TM7LN1
• NCBI Protein Information: CD97 antigen
• UniProt Protein Name: CD97 antigen
• Protein Family: CD97 antigen
• UniProt Gene Name: CD97

NCBI Description

This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]

Uniprot Description

CD97: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 2; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: focal adhesion; integral to plasma membrane; membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: cell motility; cell-cell signaling; immune response; inflammatory response