Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Plexin-A1 (PLXNA1) Standard Curve.)
Mouse Plexin-A1 (PLXNA1) ELISA Kit | PLXNA1 elisa kit
Mouse Plexin-A1 (PLXNA1) ELISA Kit
Gene Names
Plxna1; NOV; Plxn1; PlexA1; mKIAA4053; 2600013D04Rik
Reactivity
Mouse
Synonyms
Plexin-A1 (PLXNA1); Mouse Plexin-A1 (PLXNA1) ELISA Kit; PLXNA1; DKFZp761P19121; NOV; NOVP; PLEXIN-A1; PLXN1; plexin 1; PLXNA1 elisa kit
Reactivity
Mouse
Specificity
Mouse Plexin-A1 (PLXNA1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLXNA1. No significant cross-reactivity or interference between Mouse PLXNA1 and analogues was observed.
Sequence Length
1894
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Plexin-A1 (PLXNA1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Plexin-A1 (PLXNA1) Standard Curve.)
Related Product Information for PLXNA1 elisa kit
Description: This Mouse Plexin-A1 (PLXNA1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLXNA1 in samples. An antibody specific for PLXNA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLXNA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PLXN1 alone did not bind semaphorin-3A (SEMA3A), but the NRP1/PLXN1 complex had a higher affinity for SEMA3A than did NRP1 alone. While SEMA3A binding to NRP1 did not alter nonneuronal cell morphology, SEMA3A interaction with NRP1/PLXN1 complexes induced adherent cells to round up. Expression of a dominant-negative PLXN1 in sensory neurons blocked SEMA3A-induced growth cone collapse. SEMA3A treatment led to the redistribution of growth cone NRP1 and PLXN1 into clusters. Thus, the authors concluded that physiologic SEMA3A receptors consist of NRP1/PLXN1 complexes.The transmembrane protein they called NOV was identified by a cDNA found in a skeletal muscle cDNA library and shares 72% identity with SEX.
Background: PLXN1 alone did not bind semaphorin-3A (SEMA3A), but the NRP1/PLXN1 complex had a higher affinity for SEMA3A than did NRP1 alone. While SEMA3A binding to NRP1 did not alter nonneuronal cell morphology, SEMA3A interaction with NRP1/PLXN1 complexes induced adherent cells to round up. Expression of a dominant-negative PLXN1 in sensory neurons blocked SEMA3A-induced growth cone collapse. SEMA3A treatment led to the redistribution of growth cone NRP1 and PLXN1 into clusters. Thus, the authors concluded that physiologic SEMA3A receptors consist of NRP1/PLXN1 complexes.The transmembrane protein they called NOV was identified by a cDNA found in a skeletal muscle cDNA library and shares 72% identity with SEX.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
plexin-A1
NCBI Official Synonym Full Names
plexin A1
NCBI Official Symbol
Plxna1
NCBI Official Synonym Symbols
NOV; Plxn1; PlexA1; mKIAA4053; 2600013D04Rik
NCBI Protein Information
plexin-A1
UniProt Protein Name
Plexin-A1
Protein Family
UniProt Gene Name
Plxna1
UniProt Synonym Gene Names
Kiaa4053; Plex 1; Plexin-1
Uniprot Description
Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
Research Articles on PLXNA1
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Product Notes
The Mouse PLXNA1 plxna1 (Catalog #AAA9717331) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717331 ELISA Kit recognizes Mouse PLXNA1. It is sometimes possible for the material contained within the vial of "Plexin-A1 (PLXNA1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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