Human Carnitine Acetyltransferase (CRAT) ELISA Kit | CRAT elisa kit

Human Carnitine Acetyltransferase (CRAT) ELISA Kit

Cat. Num. AAA2704415

• Gene Names: CRAT; CAT; CAT1
• Reactivity: Human
• Synonyms: Carnitine Acetyltransferase (CRAT); Human Carnitine Acetyltransferase (CRAT) ELISA Kit; CAT1; Carnitine O-Acetyltransferase; Carnitine acetylase; CRAT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Carnitine Acetyltransferase (CRAT).; No significant cross-reactivity or interference between Carnitine Acetyltransferase (CRAT) and analogues was observed.
• Sequence Length: 544

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnitine Acetyltransferase (CRAT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnitine Acetyltransferase (CRAT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRAT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Acetyltransferase (CRAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carnitine Acetyltransferase (CRAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnitine Acetyltransferase (CRAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carnitine Acetyltransferase (CRAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 927415
• NCBI GeneID: 1384
• UniProt Accession #: P43155; Q5T952; Q9BW16
• Molecular Weight: 61,870 Da
• NCBI Official Full Name: carnitine acetyltransferase, partial
• NCBI Official Synonym Full Names: carnitine O-acetyltransferase
• NCBI Official Symbol: CRAT
• NCBI Official Synonym Symbols: CAT; CAT1
• NCBI Protein Information: carnitine O-acetyltransferase
• UniProt Protein Name: Carnitine O-acetyltransferase
• Protein Family: Carnitine O-acetyltransferase
• UniProt Gene Name: CRAT
• UniProt Synonym Gene Names: CAT1; Carnitine acetylase; CAT; CrAT

NCBI Description

This gene encodes carnitine O-acetyltransferase, a member of the carnitine acyltransferase family and a key metabolic pathway enzyme which plays an important role in energy homeostasis and fat metabolism. This enzyme catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acyl-CoA/CoA. It is found in both the mitochondria and the peroxisome. Alternative splicing results in transcript variants encoding different isoforms that may localize to different subcellular compartments. [provided by RefSeq, Oct 2016]

Uniprot Description

Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.