Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plexin-B3 (PLXNB3) Standard Curve.)
Human Plexin-B3 (PLXNB3) ELISA Kit | PLXNB3 elisa kit
Human Plexin-B3 (PLXNB3) ELISA Kit
Gene Names
PLXNB3; PLEXR; PLXN6; PLEXB3
Reactivity
Human
Synonyms
Plexin-B3 (PLXNB3); Human Plexin-B3 (PLXNB3) ELISA Kit; PLXNB3; FLJ76953; PLEXB3; PLEXR; PLXN6; plexin 6; PLXNB3 elisa kit
Reactivity
Human
Specificity
Human Plexin-B3 (PLXNB3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLXNB3. No significant cross-reactivity or interference between Human PLXNB3 and analogues was observed.
Sequence Length
1909
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plexin-B3 (PLXNB3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plexin-B3 (PLXNB3) Standard Curve.)
Related Product Information for PLXNB3 elisa kit
Description: This Human Plexin-B3 (PLXNB3) ELISA Kit employs a two-site sandwich ELISA to quantitate PLXNB3 in samples. An antibody specific for PLXNB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLXNB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tamagnone et al. (1999) identified the cDNA sequence of a novel member of the plexin gene family, which they called plexin B3.Artigiani et al. (2004) showed that mammalian Plxnb3 is a high-affinity semaphorin-5A (SEMA5A) receptor. Plxnb3 was activated by Sema5a, and the Sema5a-Plxnb3 complex triggered intracellular signaling through Met, the receptor for hepatocyte growth factor.Tamagnone et al. (1999) found the PLXNB3 coding sequence in the genomic sequence of the ABCD1 gene on Xq28.Matsumoto et al. (2005) identified a partial deletion (exons 3 to 10) of the ABCD1 gene with fusion to the neighboring PLXNB3 gene. Sequencing showed that the breakpoints were at nucleotide +1374 in intron 2 of the ABCD1 gene and nucleotide +915 in intron 2 of the PLXNB3 gene.
Background: Tamagnone et al. (1999) identified the cDNA sequence of a novel member of the plexin gene family, which they called plexin B3.Artigiani et al. (2004) showed that mammalian Plxnb3 is a high-affinity semaphorin-5A (SEMA5A) receptor. Plxnb3 was activated by Sema5a, and the Sema5a-Plxnb3 complex triggered intracellular signaling through Met, the receptor for hepatocyte growth factor.Tamagnone et al. (1999) found the PLXNB3 coding sequence in the genomic sequence of the ABCD1 gene on Xq28.Matsumoto et al. (2005) identified a partial deletion (exons 3 to 10) of the ABCD1 gene with fusion to the neighboring PLXNB3 gene. Sequencing showed that the breakpoints were at nucleotide +1374 in intron 2 of the ABCD1 gene and nucleotide +915 in intron 2 of the PLXNB3 gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
plexin-B3 isoform 1
NCBI Official Synonym Full Names
plexin B3
NCBI Official Symbol
PLXNB3
NCBI Official Synonym Symbols
PLEXR; PLXN6; PLEXB3
NCBI Protein Information
plexin-B3
UniProt Protein Name
Plexin-B3
Protein Family
UniProt Gene Name
PLXNB3
UniProt Synonym Gene Names
KIAA1206; PLXN6
NCBI Description
The protein encoded by this gene is a member of the plexin family. It functions as a receptor for semaphorin 5A, and plays a role in axon guidance, invasive growth and cell migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Uniprot Description
Receptor for SEMA5A that plays a role in axon guidance, invasive growth and cell migration. Stimulates neurite outgrowth and mediates Ca2+/Mg2+-dependent cell aggregation. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1.
Research Articles on PLXNB3
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Product Notes
The Human PLXNB3 plxnb3 (Catalog #AAA9714866) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714866 ELISA Kit recognizes Human PLXNB3. It is sometimes possible for the material contained within the vial of "Plexin-B3 (PLXNB3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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