Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptidoglycan recognition protein 1 (PGLYRP1) Standard Curve.)
Mouse Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA Kit | PGLYRP1 elisa kit
Mouse Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA Kit
Gene Names
Pglyrp1; PGRP; Tag7; Tasg7; PGRP-S; Pglyrp; Tnfsf3l
Reactivity
Mouse
Synonyms
Peptidoglycan Recognition Protein 1 (PGLYRP1); Mouse Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA Kit; PGLYRP1; MGC126894; MGC126896; PGLYRP; PGRP; PGRP-S; PGRPS; TAG7; TNFSF3L; TNF superfamily; member 3 (LTB)-like (peptidoglycan recognition protein); PGLYRP1 elisa kit
Reactivity
Mouse
Specificity
Mouse Peptidoglycan recognition protein 1 (PGLYRP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PGLYRP1. No significant cross-reactivity or interference between Mouse PGLYRP1 and analogues was observed.
Sequence Length
182
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptidoglycan recognition protein 1 (PGLYRP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Peptidoglycan recognition protein 1 (PGLYRP1) Standard Curve.)
Related Product Information for PGLYRP1 elisa kit
Description: This Mouse Peptidoglycan recognition protein 1 (PGLYRP1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGLYRP1 in samples. An antibody specific for PGLYRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGLYRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGLYRP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGLYRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Innate nonself immune recognition relies on structures common among invading microbes, a process termed pattern recognition. Peptidoglycan is a fundamental component of the bacterial cell wall and is thus a candidate for a pattern recognized by the immune system. The deduced human PGLYRP protein has 196 amino acids. Both the human and mouse PGLYRP proteins share 43% sequence identity with the T. ni PGLYRP protein. Recombinant mouse Pglyrp protein expressed in insect cells possessed an affinity for peptidoglycan. Dot blot analysis of a number of human tissue mRNAs detected strong expression in bone marrow and weak expression in lung, kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, and fetal spleen.
Background: Innate nonself immune recognition relies on structures common among invading microbes, a process termed pattern recognition. Peptidoglycan is a fundamental component of the bacterial cell wall and is thus a candidate for a pattern recognized by the immune system. The deduced human PGLYRP protein has 196 amino acids. Both the human and mouse PGLYRP proteins share 43% sequence identity with the T. ni PGLYRP protein. Recombinant mouse Pglyrp protein expressed in insect cells possessed an affinity for peptidoglycan. Dot blot analysis of a number of human tissue mRNAs detected strong expression in bone marrow and weak expression in lung, kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, and fetal spleen.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peptidoglycan recognition protein 1
NCBI Official Synonym Full Names
peptidoglycan recognition protein 1
NCBI Official Symbol
Pglyrp1
NCBI Official Synonym Symbols
PGRP; Tag7; Tasg7; PGRP-S; Pglyrp; Tnfsf3l
NCBI Protein Information
peptidoglycan recognition protein 1
UniProt Protein Name
Peptidoglycan recognition protein 1
Protein Family
UniProt Gene Name
Pglyrp1
UniProt Synonym Gene Names
Pglyrp; Pgrp; Pgrps; Tag7; PGRP-S
Uniprot Description
Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria. Involved in innate immunity. May function in intracellular killing of bacteria. The soluble form triggers apoptosis in vitro.
Research Articles on PGLYRP1
1. Tag7 activates lymphocytes capable of Fasl-Fas-dependent contact killing of virus-infected cells.
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Product Notes
The Mouse PGLYRP1 pglyrp1 (Catalog #AAA9717369) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717369 ELISA Kit recognizes Mouse PGLYRP1. It is sometimes possible for the material contained within the vial of "Peptidoglycan Recognition Protein 1 (PGLYRP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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