Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Paired mesoderm homeobox protein 2 (PRRX2) Standard Curve.)
Mouse Paired Mesoderm Homeobox Protein 2 (PRRX2) ELISA Kit | PRRX2 elisa kit
Mouse Paired Mesoderm Homeobox Protein 2 (PRRX2) ELISA Kit
Gene Names
Prrx2; S8; Prx2
Reactivity
Mouse
Synonyms
Paired Mesoderm Homeobox Protein 2 (PRRX2); Mouse Paired Mesoderm Homeobox Protein 2 (PRRX2) ELISA Kit; PRRX2; MGC19843; PMX2; PRX2; paired mesoderm homeobox protein 2; paired-like homeodomain protein PRX2; PRRX2 elisa kit
Reactivity
Mouse
Specificity
Mouse Paired mesoderm homeobox protein 2 (PRRX2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRRX2. No significant cross-reactivity or interference between Mouse PRRX2 and analogues was observed.
Sequence Length
247
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Paired mesoderm homeobox protein 2 (PRRX2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Paired mesoderm homeobox protein 2 (PRRX2) Standard Curve.)
Related Product Information for PRRX2 elisa kit
Description: This Mouse Paired mesoderm homeobox protein 2 (PRRX2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRRX2 in samples. An antibody specific for PRRX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRRX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRRX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRRX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PRRX2 is a member of the paired family of homeobox proteins. Expression is localized to proliferating fetal fibroblasts and the developing dermal layer, with downregulated expression in adult skin. Increases in expression of this gene during fetal but not adult wound healing suggest a possible role in mechanisms that control mammalian dermal regeneration and prevent formation of scar response to wounding. The composite 813-nucleotide cDNA encodes a putative 164-amino acid protein with 74% overall identity to mouse Prx2, showing high conservation in the homeodomain and extreme 3-prime region. PRX2 is expressed as a single 1.3-kb transcript. PRX2 is more strongly expressed in normal fetal than in adult skin, much more strongly expressed in both fetal and adult proliferating fibroblasts, and not expressed in keratinocytes.
Background: PRRX2 is a member of the paired family of homeobox proteins. Expression is localized to proliferating fetal fibroblasts and the developing dermal layer, with downregulated expression in adult skin. Increases in expression of this gene during fetal but not adult wound healing suggest a possible role in mechanisms that control mammalian dermal regeneration and prevent formation of scar response to wounding. The composite 813-nucleotide cDNA encodes a putative 164-amino acid protein with 74% overall identity to mouse Prx2, showing high conservation in the homeodomain and extreme 3-prime region. PRX2 is expressed as a single 1.3-kb transcript. PRX2 is more strongly expressed in normal fetal than in adult skin, much more strongly expressed in both fetal and adult proliferating fibroblasts, and not expressed in keratinocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
paired mesoderm homeobox protein 2
NCBI Official Synonym Full Names
paired related homeobox 2
NCBI Official Symbol
Prrx2
NCBI Official Synonym Symbols
S8; Prx2
NCBI Protein Information
paired mesoderm homeobox protein 2
UniProt Protein Name
Paired mesoderm homeobox protein 2
Protein Family
UniProt Gene Name
Prrx2
UniProt Synonym Gene Names
Prx2; S8; PRX-2
UniProt Entry Name
PRRX2_MOUSE
Research Articles on PRRX2
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Product Notes
The Mouse PRRX2 prrx2 (Catalog #AAA9717252) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717252 ELISA Kit recognizes Mouse PRRX2. It is sometimes possible for the material contained within the vial of "Paired Mesoderm Homeobox Protein 2 (PRRX2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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