Rabbit anti-Mouse PRRX2 Polyclonal Antibody | anti-PRRX2 antibody

PRRX2, CT (PRRX2, PMX2, PRX2, Paired mesoderm homeobox protein 2, Paired-related homeobox protein 2) (Azide free) (HRP)

Cat. Num. AAA6337879

• Gene Names: PRRX2; PMX2; PRX2
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRRX2; Polyclonal Antibody; CT (PRRX2; PMX2; PRX2; Paired mesoderm homeobox protein 2; Paired-related homeobox protein 2) (Azide free) (HRP); Paired-related homeobox protein 2; anti-PRRX2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PRRX2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PRRX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-250 amino acids from the C-terminal region of human PRRX2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PRRX2 Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PRRX2 antibody detected the PRRX2 protein (arrow).)

Related Product Information for anti-PRRX2 antibody

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins. Expression is localized to proliferating fetal fibroblasts and the developing dermal layer, with downregulated expression in adult skin. Increases in expression of this gene during fetal but not adult wound healing suggest a possible role in mechanisms that control mammalian dermal regeneration and prevent formation of scar response to wounding. The expression patterns provide evidence consistent with a role in fetal skin development and a possible role in cellular proliferation.

Product Categories/Family for anti-PRRX2 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Eriksson, N., et al. PLoS Genet. 6 (6), E1000993 (2010): Tokutomi, Y., et al. Biochem. Biophys. Res. Commun. 364(4):822-830(2007). Wissmuller, S., et al. Nucleic Acids Res. 34(6):1735-1744(2006). Gervais, C., et al. Leukemia 19(1):145-148(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 7706659
• NCBI GeneID: 51450
• NCBI Accession #: NP_057391.1
• NCBI GenBank Nucleotide #: NM_016307.3
• UniProt Accession #: Q99811; Q5SZB5; Q9UIB3
• Molecular Weight: 27,079 Da
• NCBI Official Full Name: paired mesoderm homeobox protein 2
• NCBI Official Synonym Full Names: paired related homeobox 2
• NCBI Official Symbol: PRRX2
• NCBI Official Synonym Symbols: PMX2; PRX2
• NCBI Protein Information: paired mesoderm homeobox protein 2; PRX-2; paired-related homeobox protein 2; paired-like homeodomain protein PRX2
• UniProt Protein Name: Paired mesoderm homeobox protein 2
• Protein Family: Paired mesoderm homeobox protein
• UniProt Gene Name: PRRX2
• UniProt Synonym Gene Names: PMX2; PRX2; PRX-2
• UniProt Entry Name: PRRX2_HUMAN

NCBI Description

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins. Expression is localized to proliferating fetal fibroblasts and the developing dermal layer, with downregulated expression in adult skin. Increases in expression of this gene during fetal but not adult wound healing suggest a possible role in mechanisms that control mammalian dermal regeneration and prevent formation of scar response to wounding. The expression patterns provide evidence consistent with a role in fetal skin development and a possible role in cellular proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May play a role in the scarless healing of cutaneous wounds during the first two trimesters of development. Ref.5

Subcellular location: Nucleus

By similarity.

Tissue specificity: In fetal skin, highest expression found in cells of mesodermal origin within the dermal papilla of the developing hair shaft. Not detected in epidermis or dermis. In adult skin, weakly expressed within the basal layers of the epidermis. Not expressed in dermis. Ref.5

Developmental stage: Higher expression in fetus than in adult. Ref.5

Sequence similarities: Belongs to the paired homeobox family.Contains 1 homeobox DNA-binding domain.