Rat TBX2 ELISA Kit | TBX2 elisa kit

Rat TBX2 (T-Box Protein 2) ELISA Kit

Cat. Num. AAA2512236

• Reactivity: Rat
• Synonyms: TBX2; Rat TBX2 (T-Box Protein 2) ELISA Kit; TBX2 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat TBX2. No significant cross-reactivity or interference between Rat TBX2 and analogues was observed.
• Sequence Length: 712

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TBX2 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat TBX2 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TBX2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TBX2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TBX2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TBX2. You can calculate the concentration of TBX2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 197333868
• NCBI GeneID: 6909
• NCBI Accession #: NP_005985.3
• NCBI GenBank Nucleotide #: NM_005994.3
• UniProt Accession #: Q13207; Q16424; Q7Z647
• Molecular Weight: 75,066 Da
• NCBI Official Full Name: T-box transcription factor TBX2
• NCBI Official Synonym Full Names: T-box 2
• NCBI Official Symbol: TBX2
• NCBI Protein Information: T-box transcription factor TBX2; T-box protein 2
• UniProt Protein Name: T-box transcription factor TBX2
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX2
• UniProt Synonym Gene Names: T-box protein 2
• UniProt Entry Name: TBX2_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product is the human homolog of mouse Tbx2, and shares strong sequence similarity with Drosophila omb protein. Expression studies indicate that this gene may have a potential role in tumorigenesis as an immortalizing agent. Transcript heterogeneity due to alternative polyadenylation has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TBX2: Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: transcription factor complex; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding

Biological Process: cardiac muscle development; transcription, DNA-dependent; positive regulation of cell proliferation; cell aging; embryonic digit morphogenesis; palate development; embryonic camera-type eye morphogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; muscle cell fate determination; positive regulation of cardiac muscle cell proliferation