Rabbit PLSCR4 Polyclonal Antibody | anti-PLSCR4 antibody

PLSCR4, ID (PLSCR4, Phospholipid scramblase 4, Ca(2+)-dependent phospholipid scramblase 4, Cell growth-inhibiting gene 43 protein, TRA1) (Biotin)

Cat. Num. AAA6335094

• Gene Names: PLSCR4; TRA1
• Reactivity: Human; Predicted: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PLSCR4; Polyclonal Antibody; ID (PLSCR4; Phospholipid scramblase 4; Ca(2+)-dependent phospholipid scramblase 4; Cell growth-inhibiting gene 43 protein; TRA1) (Biotin); TRA1; anti-PLSCR4 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 329

• Applicable Applications for anti-PLSCR4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PLSCR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-151 amino acids from the Central region of human PLSCR4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PLSCR4 Antibody (Center) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the PLSCR4 antibody detected the PLSCR4 protein (arrow).)

Immunohistochemistry (IHC)

(PLSCR4 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLSCR4 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PLSCR4 antibody

PLSCR4 may mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.

Product Categories/Family for anti-PLSCR4 antibody

Antibodies; Enzymes

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Sahu, S.K., et al. Biochim. Biophys. Acta 1790(10):1274-1281(2009). Py, B., et al. PLoS ONE 4 (3), E5006 (2009): Shibata, H., et al. J. Biol. Chem. 283(15):9623-9632(2008). Wiedmer, T., et al. Biochim. Biophys. Acta 1467(1):244-253(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 190194377
• NCBI GeneID: 57088
• NCBI Accession #: NP_065086.2; NP_001121778.1; NP_001170775.1
• NCBI GenBank Nucleotide #: NP_065086.2
• UniProt Accession #: Q9NRQ2
• NCBI Official Full Name: phospholipid scramblase 4 isoform a
• NCBI Official Synonym Full Names: phospholipid scramblase 4
• NCBI Official Symbol: PLSCR4
• NCBI Official Synonym Symbols: TRA1
• NCBI Protein Information: phospholipid scramblase 4
• UniProt Protein Name: Phospholipid scramblase 4
• Protein Family: Phospholipid scramblase
• UniProt Gene Name: PLSCR4
• UniProt Synonym Gene Names: PL scramblase 4

Uniprot Description

PLSCR4: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Belongs to the phospholipid scramblase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: integral component of membrane; plasma membrane

Molecular Function: calcium ion binding; CD4 receptor binding; enzyme binding; phospholipid scramblase activity; protein binding; SH3 domain binding

Biological Process: phospholipid scrambling