Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myosin-4 (MYH4) Standard Curve.)
Mouse Myosin-4 (MYH4) ELISA Kit | MYH4 elisa kit
Mouse Myosin-4 (MYH4) ELISA Kit
Gene Names
Myh4; MM; MHC2B; Myhsf; MYH-2B; Minmus; Minimsc; AI506973; MyHC-IIb
Reactivity
Mouse
Synonyms
Myosin-4 (MYH4); Mouse Myosin-4 (MYH4) ELISA Kit; MYH4; MYH2B; MyHC-2B; MyHC-IIb; myosin; heavy polypeptide 4; skeletal muscle; MYH4 elisa kit
Reactivity
Mouse
Specificity
Mouse Myosin-4 (MYH4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYH4. No significant cross-reactivity or interference between Mouse MYH4 and analogues was observed.
Sequence Length
1939
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myosin-4 (MYH4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myosin-4 (MYH4) Standard Curve.)
Related Product Information for MYH4 elisa kit
Description: This Mouse Myosin-4 (MYH4) ELISA Kit employs a two-site sandwich ELISA to quantitate MYH4 in samples. An antibody specific for MYH4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYH4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYH4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYH4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: All MHC proteins share approximately 100% conservation in the phosphate-binding loop, several helices forming the nucleotide-binding pocket, residues involved in actin binding, residues involved in the stereospecific hydrophobic rigor state interaction of actin with myosin, a cleft that divides the ATP- and actin-binding sites, and a converter domain, or fulcrum, containing 2 conserved cysteines.the MYH4 gene would appear to be located on 17p13.1, the location of 4 other myosin heavy chain genes (MYH1; MYH2; MYH3; and MYH8). Furthermore, Soussi-Yanicostas et al. (1993) had evidence from analysis of YACs that the embryonic and fetal genes, on the one hand, and the adult fast myosin heavy chain genes, on the other hand, are contained within different genomic fragments.
Background: All MHC proteins share approximately 100% conservation in the phosphate-binding loop, several helices forming the nucleotide-binding pocket, residues involved in actin binding, residues involved in the stereospecific hydrophobic rigor state interaction of actin with myosin, a cleft that divides the ATP- and actin-binding sites, and a converter domain, or fulcrum, containing 2 conserved cysteines.the MYH4 gene would appear to be located on 17p13.1, the location of 4 other myosin heavy chain genes (MYH1; MYH2; MYH3; and MYH8). Furthermore, Soussi-Yanicostas et al. (1993) had evidence from analysis of YACs that the embryonic and fetal genes, on the one hand, and the adult fast myosin heavy chain genes, on the other hand, are contained within different genomic fragments.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myosin-4
NCBI Official Synonym Full Names
myosin, heavy polypeptide 4, skeletal muscle
NCBI Official Symbol
Myh4
NCBI Official Synonym Symbols
MM; MHC2B; Myhsf; MYH-2B; Minmus; Minimsc; AI506973; MyHC-IIb
NCBI Protein Information
myosin-4
UniProt Protein Name
Myosin-4
Protein Family
UniProt Gene Name
Myh4
UniProt Synonym Gene Names
MyHC-2b
UniProt Entry Name
MYH4_MOUSE
Uniprot Description
MYH4: Muscle contraction.
Protein type: Motor; Motility/polarity/chemotaxis
Cellular Component: cytoplasm; myofibril; myosin complex
Molecular Function: actin binding; ATP binding; calmodulin binding; double-stranded RNA binding; motor activity; nucleotide binding
Biological Process: muscle contraction; response to activity
Research Articles on MYH4
Similar Products
Product Notes
The Mouse MYH4 myh4 (Catalog #AAA9717989) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717989 ELISA Kit recognizes Mouse MYH4. It is sometimes possible for the material contained within the vial of "Myosin-4 (MYH4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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