Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myosin-Va (MYO5A) Standard Curve.)
Human Myosin-Va (MYO5A) ELISA Kit | MYO5A elisa kit
Human Myosin-Va (MYO5A) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myosin-Va (MYO5A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myosin-Va (MYO5A) Standard Curve.)
Background: MYO5A is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. Myosin-Va encoded by MYO5A is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined.
NCBI and Uniprot Product Information
NCBI Description
This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]
Uniprot Description
MYO5A: Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. May also be required for some polarization process involved in dendrite formation. May be a homodimer, which associates with multiple calmodulin or myosin light chains. Binds MLPH and MYRIP. Interacts with RIPL2, the interaction is required for its role in dendrite formation. Detected in melanocytes. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 15q21
Cellular Component: Golgi apparatus; filopodium tip; neuron projection; lysosome; endoplasmic reticulum; early endosome; intermediate filament; actomyosin; actin filament; cytosol; ruffle; recycling endosome; photoreceptor outer segment; growth cone; cell soma; membrane; late endosome; cytoplasm; melanosome; peroxisome; myosin complex; vesicle
Molecular Function: microfilament motor activity; calmodulin binding; calcium ion binding; actin binding; Rab GTPase binding; ATP binding
Biological Process: myelination; exocytosis; ER localization; actin filament-based movement; long-chain fatty acid biosynthetic process; melanosome transport; locomotion during locomotory behavior; odontogenesis; vesicle-mediated transport; melanin biosynthetic process; vesicle transport along actin filament; synaptic transmission; cellular protein metabolic process; cellular response to insulin stimulus; visual perception; insulin secretion; transport; melanocyte differentiation; synapse organization and biogenesis; regulation of inositol-1,4,5-triphosphate receptor activity; post-Golgi vesicle-mediated transport; secretory granule localization
Disease: Griscelli Syndrome, Type 1
Research Articles on MYO5A
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Product Notes
The Human MYO5A myo5a (Catalog #AAA9715080) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715080 ELISA Kit recognizes Human MYO5A. It is sometimes possible for the material contained within the vial of "Myosin-Va (MYO5A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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