Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myosin-If (MYO1F) Standard Curve.)
Human Myosin-If (MYO1F) ELISA Kit | MYO1F elisa kit
Human Myosin-If (MYO1F) ELISA Kit
Reactivity
Human
Synonyms
Myosin-If (MYO1F); Human Myosin-If (MYO1F) ELISA Kit; MYO1F; MYO1F variant protein; myosin-ID; MYO1F elisa kit
Reactivity
Human
Specificity
Human Myosin-If (MYO1F) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO1F. No significant cross-reactivity or interference between Human MYO1F and analogues was observed.
Sequence Length
1098
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myosin-If (MYO1F) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myosin-If (MYO1F) Standard Curve.)
Related Product Information for MYO1F elisa kit
Description: This Human Myosin-If (MYO1F) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1F in samples. An antibody specific for MYO1F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO1F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO1F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO1F bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Myosins are molecular motors that use the energy from ATP hydrolysis to generate force on actin filaments. Myosin-If encoded by MYO1F is an unconventional myosin that may be involved in the intracellular movement of membrane-enclosed compartments. There is evidence to suggest that mutations in MYO1F can result in hearing loss.
Background: Myosins are molecular motors that use the energy from ATP hydrolysis to generate force on actin filaments. Myosin-If encoded by MYO1F is an unconventional myosin that may be involved in the intracellular movement of membrane-enclosed compartments. There is evidence to suggest that mutations in MYO1F can result in hearing loss.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Myosin IF
NCBI Official Synonym Full Names
myosin IF
NCBI Official Symbol
MYO1F
NCBI Protein Information
unconventional myosin-If
UniProt Protein Name
Unconventional myosin-If
Protein Family
UniProt Gene Name
MYO1F
UniProt Entry Name
MYO1F_HUMAN
NCBI Description
Myosins are molecular motors that use the energy from ATP hydrolysis to generate force on actin filaments. The protein encoded by this gene is an unconventional myosin that may be involved in the intracellular movement of membrane-enclosed compartments. There is evidence to suggest that mutations in this gene can result in hearing loss. [provided by RefSeq, Jan 2017]
Research Articles on MYO1F
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Product Notes
The Human MYO1F myo1f (Catalog #AAA9715085) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715085 ELISA Kit recognizes Human MYO1F. It is sometimes possible for the material contained within the vial of "Myosin-If (MYO1F), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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