Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Soluble myosin heavy chain 1 (sMHC-1) Standard Curve.)
Human Soluble Myosin Heavy Chain 1 (sMHC-1) ELISA Kit | sMHC-1 elisa kit
Human Soluble Myosin Heavy Chain 1 (sMHC-1) ELISA Kit
Gene Names
MYH1; MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
Reactivity
Human
Synonyms
Soluble Myosin Heavy Chain 1 (sMHC-1); Human Soluble Myosin Heavy Chain 1 (sMHC-1) ELISA Kit; MYH1; MGC133384; MYHSA1; MYHa; MyHC-2X/D; myosin heavy chainx/d; myosin; heavy polypeptide 1; skeletal muscle; adult; sMHC-1 elisa kit
Reactivity
Human
Specificity
Human Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYH1. No significant cross-reactivity or interference between Human MYH1 and analogues was observed.
Sequence Length
1939
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Soluble myosin heavy chain 1 (sMHC-1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Soluble myosin heavy chain 1 (sMHC-1) Standard Curve.)
Related Product Information for sMHC-1 elisa kit
Description: This Human Soluble myosin heavy chain 1 (sMHC-1) ELISA Kit employs a two-site sandwich ELISA to quantitate MYH1 in samples. An antibody specific for MYH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
Background: Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myosin-1
NCBI Official Synonym Full Names
myosin heavy chain 1
NCBI Official Symbol
MYH1
NCBI Official Synonym Symbols
MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
NCBI Protein Information
myosin-1
UniProt Protein Name
Myosin-1
UniProt Gene Name
MYH1
UniProt Synonym Gene Names
MyHC-2x; MyHC-IIx/d
UniProt Entry Name
MYH1_HUMAN
NCBI Description
Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]
Uniprot Description
MYH1: Muscle contraction.
Protein type: Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: muscle myosin complex; A band
Molecular Function: calmodulin binding; motor activity; actin binding; ATP binding
Biological Process: muscle contraction; metabolic process
Research Articles on sMHC-1
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Product Notes
The Human sMHC-1 myh1 (Catalog #AAA9715111) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715111 ELISA Kit recognizes Human sMHC-1. It is sometimes possible for the material contained within the vial of "Soluble Myosin Heavy Chain 1 (sMHC-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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