Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myelin transcription factor 1-like protein (MYT1L) Standard Curve.)
Mouse Myelin Transcription Factor 1-Like Protein (MYT1L) ELISA Kit | MYT1L elisa kit
Mouse Myelin Transcription Factor 1-Like Protein (MYT1L) ELISA Kit
Reactivity
Mouse
Synonyms
Myelin Transcription Factor 1-Like Protein (MYT1L); Mouse Myelin Transcription Factor 1-Like Protein (MYT1L) ELISA Kit; MYT1L; NZF1; neural zinc finger transcription factor 1; MYT1L elisa kit
Reactivity
Mouse
Specificity
Mouse Myelin transcription factor 1-like protein (MYT1L) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYT1L. No significant cross-reactivity or interference between Mouse MYT1L and analogues was observed.
Sequence Length
1187
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myelin transcription factor 1-like protein (MYT1L) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myelin transcription factor 1-like protein (MYT1L) Standard Curve.)
Related Product Information for MYT1L elisa kit
Description: This Mouse Myelin transcription factor 1-like protein (MYT1L) ELISA Kit employs a two-site sandwich ELISA to quantitate MYT1L in samples. An antibody specific for MYT1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYT1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYT1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYT1L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Transcription factor that plays a key role in neuronal differentiation by specifically repressing expression of non-neuronal genes during neuron differentiation. In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs. Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B. The 5'-AAGTT-3' core motif is absent from the promoter of neural genes.
Background: Transcription factor that plays a key role in neuronal differentiation by specifically repressing expression of non-neuronal genes during neuron differentiation. In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs. Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B. The 5'-AAGTT-3' core motif is absent from the promoter of neural genes.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Myelin transcription factor 1-like protein
UniProt Protein Name
Myelin transcription factor 1-like protein
Protein Family
UniProt Gene Name
Myt1l
UniProt Synonym Gene Names
Kiaa1106; Nzf1; Png1; MyT1-L; MyT1L; NZF-1
UniProt Entry Name
MYT1L_MOUSE
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Product Notes
The Mouse MYT1L myt1l (Catalog #AAA9717457) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717457 ELISA Kit recognizes Mouse MYT1L. It is sometimes possible for the material contained within the vial of "Myelin Transcription Factor 1-Like Protein (MYT1L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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