Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human RE1-silencing transcription factor (REST) Standard Curve.)
Human RE1-Silencing Transcription Factor (REST) ELISA Kit | REST elisa kit
Human RE1-Silencing Transcription Factor (REST) ELISA Kit
Gene Names
REST; WT6; XBR; HGF5; NRSF; DFNA27; GINGF5
Reactivity
Human
Synonyms
RE1-Silencing Transcription Factor (REST); Human RE1-Silencing Transcription Factor (REST) ELISA Kit; REST; NRSF; XBR; neuron restrictive silencer factor; repressor binding to the X2 box; REST elisa kit
Reactivity
Human
Specificity
Human RE1-silencing transcription factor (REST) ELISA Kit has high sensitivity and excellent specificity for detection of Human REST. No significant cross-reactivity or interference between Human REST and analogues was observed.
Sequence Length
1097
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human RE1-silencing transcription factor (REST) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human RE1-silencing transcription factor (REST) Standard Curve.)
Related Product Information for REST elisa kit
Description: This Human RE1-silencing transcription factor (REST) ELISA Kit employs a two-site sandwich ELISA to quantitate REST in samples. An antibody specific for REST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined.
Background: This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
RE1-silencing transcription factor
NCBI Official Synonym Full Names
RE1 silencing transcription factor
NCBI Official Symbol
REST
NCBI Official Synonym Symbols
WT6; XBR; HGF5; NRSF; DFNA27; GINGF5
NCBI Protein Information
RE1-silencing transcription factor
UniProt Protein Name
RE1-silencing transcription factor
Protein Family
UniProt Gene Name
REST
UniProt Synonym Gene Names
NRSF; XBR
UniProt Entry Name
REST_HUMAN
NCBI Description
This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018]
Research Articles on REST
Similar Products
Product Notes
The Human REST rest (Catalog #AAA9714470) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714470 ELISA Kit recognizes Human REST. It is sometimes possible for the material contained within the vial of "RE1-Silencing Transcription Factor (REST), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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