Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myelin basic protein (MBP) Standard Curve.)
Rat Myelin Basic Protein (MBP) ELISA Kit | MBP elisa kit
Rat Myelin Basic Protein (MBP) ELISA Kit
Reactivity
Rat
Synonyms
Myelin Basic Protein (MBP); Rat Myelin Basic Protein (MBP) ELISA Kit; MBP; MGC99675; Golli-mbp; OTTHUMP00000174383; MBP elisa kit
Reactivity
Rat
Specificity
Rat Myelin basic protein (MBP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MBP. No significant cross-reactivity or interference between Rat MBP and analogues was observed.
Sequence Length
168
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myelin basic protein (MBP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Myelin basic protein (MBP) Standard Curve.)
Related Product Information for MBP elisa kit
Description: This Rat Myelin basic protein (MBP) ELISA Kit employs a two-site sandwich ELISA to quantitate MBP in samples. An antibody specific for MBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the central nervous system (CNS). Myelin Basic Protein (MBP) is the major constituent of the CNS myelin synthesized by oligodendrocytes and Schwann cells. MBP, PLP (proteolipid proteins) and MOG (myelin oligodendrocyte glycoprotein) are potential primary encephalitogenic target antigens in multiple sclerosis.MBP was initially sequenced in 1979 after isolation from myelin membranes. Since that time, knockout mice deficient in MBP have been developed which showed decreased amounts of CNS myelination and a progressive disorder characterized by tremors, seizures, and early death. The gene for MBP is on chromosome 18; the protein localizes to the CNS and to various cells of the hematopoietic system.
Background: Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the central nervous system (CNS). Myelin Basic Protein (MBP) is the major constituent of the CNS myelin synthesized by oligodendrocytes and Schwann cells. MBP, PLP (proteolipid proteins) and MOG (myelin oligodendrocyte glycoprotein) are potential primary encephalitogenic target antigens in multiple sclerosis.MBP was initially sequenced in 1979 after isolation from myelin membranes. Since that time, knockout mice deficient in MBP have been developed which showed decreased amounts of CNS myelination and a progressive disorder characterized by tremors, seizures, and early death. The gene for MBP is on chromosome 18; the protein localizes to the CNS and to various cells of the hematopoietic system.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Myelin basic protein
UniProt Protein Name
Myelin basic protein
Protein Family
UniProt Gene Name
MBP
UniProt Synonym Gene Names
MBP
Uniprot Description
Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane ().
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Product Notes
The Rat MBP mbp (Catalog #AAA9712312) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712312 ELISA Kit recognizes Rat MBP. It is sometimes possible for the material contained within the vial of "Myelin Basic Protein (MBP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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