Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melatonin receptor type 1A (MTNR1A) Standard Curve.)
Mouse Melatonin Receptor Type 1A (MTNR1A) ELISA Kit | MTNR1A elisa kit
Mouse Melatonin Receptor Type 1A (MTNR1A) ELISA Kit
Gene Names
Mtnr1a; MR; MelR
Reactivity
Mouse
Synonyms
Melatonin Receptor Type 1A (MTNR1A); Mouse Melatonin Receptor Type 1A (MTNR1A) ELISA Kit; MTNR1A; MEL-1A-R; MT1; melatonin receptor type 1A; MTNR1A elisa kit
Reactivity
Mouse
Specificity
Mouse Melatonin receptor type 1A (MTNR1A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MTNR1A. No significant cross-reactivity or interference between Mouse MTNR1A and analogues was observed.
Sequence Length
353
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melatonin receptor type 1A (MTNR1A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Melatonin receptor type 1A (MTNR1A) Standard Curve.)
Related Product Information for MTNR1A elisa kit
Description: This Mouse Melatonin receptor type 1A (MTNR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MTNR1A in samples. An antibody specific for MTNR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTNR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTNR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTNR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Mtnr1a encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.
Background: Mtnr1a encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
melatonin receptor type 1A
NCBI Official Synonym Full Names
melatonin receptor 1A
NCBI Official Symbol
Mtnr1a
NCBI Official Synonym Symbols
MR; MelR
NCBI Protein Information
melatonin receptor type 1A
UniProt Protein Name
Melatonin receptor type 1A
Protein Family
UniProt Gene Name
Mtnr1a
UniProt Synonym Gene Names
Mel-1A-R; Mel1a receptor
UniProt Entry Name
MTR1A_MOUSE
Uniprot Description
MTNR1A: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: membrane; cell soma; integral to plasma membrane; integral to membrane; plasma membrane; receptor complex
Molecular Function: G-protein coupled receptor activity; signal transducer activity; hormone binding; melatonin receptor activity
Biological Process: circadian rhythm; G-protein coupled receptor protein signaling pathway; positive regulation of cGMP biosynthetic process; signal transduction; negative regulation of insulin secretion
Research Articles on MTNR1A
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Product Notes
The Mouse MTNR1A mtnr1a (Catalog #AAA9717521) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717521 ELISA Kit recognizes Mouse MTNR1A. It is sometimes possible for the material contained within the vial of "Melatonin Receptor Type 1A (MTNR1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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