Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Melatonin (MT) Standard Curve.)
Rat Melatonin (MT) ELISA Kit | MT elisa kit
Rat Melatonin (MT) ELISA Kit
Reactivity
Rat
Synonyms
Melatonin (MT); Rat Melatonin (MT) ELISA Kit; MT elisa kit
Reactivity
Rat
Specificity
Rat Melatonin (MT) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MT. No significant cross-reactivity or interference between Rat MT and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Melatonin (MT) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Melatonin (MT) Standard Curve.)
Related Product Information for MT elisa kit
Description: This Rat Melatonin (MT) ELISA Kit employs a two-site sandwich ELISA to quantitate MT in samples. An antibody specific for MT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Melatonin, 5-methoxy-N-acetyltryptamine, is a hormone found in all living creatures from algae to humans, at levels that vary in a daily cycle. Many biological effects of melatonin are produced through activation of melatonin receptors,while others are due to its role as a pervasive and extremely powerful antioxidant with a particular role in the protection of nuclear and mitochondrial DNA. Melatonin is secreted by the pineal gland in the brain and is important in the regulation of many hormones in the body. Among its key roles, melatonin controls the body's circadian rhythm, an internal 24-hour time-keeping system that plays an important role in when fall asleep and when wake up. Darkness stimulates the release of melatonin and light suppresses its activity.
Background: Melatonin, 5-methoxy-N-acetyltryptamine, is a hormone found in all living creatures from algae to humans, at levels that vary in a daily cycle. Many biological effects of melatonin are produced through activation of melatonin receptors,while others are due to its role as a pervasive and extremely powerful antioxidant with a particular role in the protection of nuclear and mitochondrial DNA. Melatonin is secreted by the pineal gland in the brain and is important in the regulation of many hormones in the body. Among its key roles, melatonin controls the body's circadian rhythm, an internal 24-hour time-keeping system that plays an important role in when fall asleep and when wake up. Darkness stimulates the release of melatonin and light suppresses its activity.
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Product Notes
The Rat MT (Catalog #AAA9712508) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712508 ELISA Kit recognizes Rat MT. It is sometimes possible for the material contained within the vial of "Melatonin (MT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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