Bovine Orexins ELISA Kit | Orexins elisa kit

Bovine Orexins ELISA Kit

Cat. Num. AAA736844

• Gene Names: HCRT; OX; PPOX; NRCLP1
• Reactivity: Bovine
• Synonyms: Orexins; Bovine Orexins ELISA Kit; Orexins elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ORX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ORX. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Orexins elisa kit

Principle of the assay: ORX ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ORX. Standards or samples are then added to the microtiter plate wells and ORX if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ORX present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ORX are added to each well to "sandwich" the ORX immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ORX and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ORX concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Orexins elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 4557635
• NCBI GeneID: 3060
• NCBI Accession #: NP_001515.1
• NCBI GenBank Nucleotide #: NM_001524.1
• Molecular Weight: 13,363 Da
• NCBI Official Full Name: orexin
• NCBI Official Synonym Full Names: hypocretin (orexin) neuropeptide precursor
• NCBI Official Symbol: HCRT
• NCBI Official Synonym Symbols: OX; PPOX; NRCLP1
• NCBI Protein Information: orexin; prepro-orexin
• UniProt Protein Name: Orexin
• UniProt Gene Name: HCRT
• UniProt Synonym Gene Names: OX; PPORX; PPOX; Hcrt; Hcrt1; Hcrt2
• UniProt Entry Name: OREX_HUMAN

NCBI Description

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]

Uniprot Description

HCRT: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1). Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Belongs to the orexin family.

Protein type: Hormone

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: synaptic vesicle; rough endoplasmic reticulum; perinuclear region of cytoplasm; extracellular region; cell junction; secretory granule

Molecular Function: type 2 hypocretin receptor binding; type 1 hypocretin receptor binding

Biological Process: eating behavior; negative regulation of potassium ion transport; regulation of neurotransmitter secretion; synaptic transmission; elevation of cytosolic calcium ion concentration; negative regulation of transmission of nerve impulse; neuropeptide signaling pathway; negative regulation of DNA replication; protein kinase C activation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of calcium ion transport; regulation of excitatory postsynaptic membrane potential; positive regulation of transmission of nerve impulse

Disease: Narcolepsy 1