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SDS-Page

CD2 Associated Protein (CD2AP) Recombinant Protein | CD2AP recombinant protein

Recombinant CD2 Associated Protein (CD2AP)

Gene Names
Cd2ap; CMS
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
CD2 Associated Protein (CD2AP); Recombinant CD2 Associated Protein (CD2AP); CD2AP recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-DRD EKSLLEQKPS KPAAPQVPPK KPTPPTKANN LLRSPGTMYP KRPEKPVPPP PPTAKINGEV STISSKIDTE PLSKPKLDPE QLPVRPKSVD LDALVARNSK ETDNVNFDDI ASSENLLHLT ANRPKMPGRR LPGRFNGGHS PTQSPEKTLK LPKDDDSGNI KPSEFKKDAG YSSKPSLSAP SSASKVNTAA FLSPLELKAK VEADDGTKSS LDELRAQITE LLCIVDALKK DHGKELEKLR RELEEEKAMR SNL
Sequence Length
637
Applicable Applications for CD2AP recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Asp368~Leu623 (Accession # Q80ZE7) with two N-terminal Tags, His-tag and T7-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31.8kDa
NCBI Official Full Name
CD2-associated protein
NCBI Official Synonym Full Names
CD2-associated protein
NCBI Official Symbol
Cd2ap
NCBI Official Synonym Symbols
CMS
NCBI Protein Information
CD2-associated protein
UniProt Protein Name
CD2AP/CMS
Protein Family
UniProt Gene Name
Cd2ap
UniProt Entry Name
Q80ZE7_RAT

Uniprot Description

CD2AP: an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Required for cytokinesis. Links CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. Attaches to membrane proteins via its three SH3 domains and attaches to and rearranges the cytoskeleton via its C-terminus. CD2AP?s interactions with the cytoskeleton result in receptor clustering and endocysosis as well as targeting proteins to degradative vesicles in the kidney. Mice carrying a heterozygous knockout of CD2AP show defects in kidney filtration, and mutations in the human gene are associated with focal segmental glomerulosclerosis (FSGS3).

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Cellular Component: filamentous actin; ruffle; protein complex; cytoskeleton; perinuclear region of cytoplasm; endocytic vesicle; leading edge; cytoplasm; nucleolus; plasma membrane; intercellular junction

Molecular Function: protein C-terminus binding; protein binding; cadherin binding; beta-catenin binding; protein complex binding; vascular endothelial growth factor receptor binding; SH3 domain binding

Biological Process: mitosis; proteasomal ubiquitin-dependent protein catabolic process; cell-cell adhesion; cell migration; cell division; vesicle organization and biogenesis; regulation of receptor-mediated endocytosis; negative regulation of transforming growth factor-beta1 production

Research Articles on CD2AP

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Product Notes

The CD2AP cd2ap (Catalog #AAA2010335) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD2 Associated Protein (CD2AP) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CD2AP cd2ap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-DRD EKSLLEQKPS KPAAPQVPPK KPTPPTKANN LLRSPGTMYP KRPEKPVPPP PPTAKINGEV STISSKIDTE PLSKPKLDPE QLPVRPKSVD LDALVARNSK ETDNVNFDDI ASSENLLHLT ANRPKMPGRR LPGRFNGGHS PTQSPEKTLK LPKDDDSGNI KPSEFKKDAG YSSKPSLSAP SSASKVNTAA FLSPLELKAK VEADDGTKSS LDELRAQITE LLCIVDALKK DHGKELEKLR RELEEEKAMR SNL. It is sometimes possible for the material contained within the vial of "CD2 Associated Protein (CD2AP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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