Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Meiotic recombination protein SPO11 (SPO11) Standard Curve.)
Mouse Meiotic Recombination Protein SPO11 (SPO11) ELISA Kit | SPO11 elisa kit
Mouse Meiotic Recombination Protein SPO11 (SPO11) ELISA Kit
Gene Names
Spo11; Spo11a; Spo11b; AI449549
Reactivity
Mouse
Synonyms
Meiotic Recombination Protein SPO11 (SPO11); Mouse Meiotic Recombination Protein SPO11 (SPO11) ELISA Kit; SPO11; CT35; MGC39953; SPO11 meiotic protein covalently bound to DSB-like; meiotic protein covalently bound to DSB-like; cancer/testis antigen 35; meiotic recombination protein SPO11; SPO11 elisa kit
Reactivity
Mouse
Specificity
Mouse Meiotic recombination protein SPO11 (SPO11) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPO11. No significant cross-reactivity or interference between Mouse SPO11 and analogues was observed.
Sequence Length
371
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Meiotic recombination protein SPO11 (SPO11) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Meiotic recombination protein SPO11 (SPO11) Standard Curve.)
Related Product Information for SPO11 elisa kit
Description: This Mouse Meiotic recombination protein SPO11 (SPO11) ELISA Kit employs a two-site sandwich ELISA to quantitate SPO11 in samples. An antibody specific for SPO11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPO11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPO11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPO11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SPO11 is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. The human and the mouse SPO11 proteins share 82% amino acid identity, but only 20 to 30% with other eukaryotic homologs such as Drosophila and C. elegans. Both the mouse and human SPO11 exons 2 and 8 are subject to alternative splicing. Northern blot analysis showed that mouse Spo11 is expressed in testis and thymus as a 1.8-kb mRNA, whereas human SPO11 is expressed in testis as a 2.0-kb transcript. RT-PCR revealed that human SPO11 is also expressed in prostate, fetal testis, thymus, and some carcinoma cell lines.
Background: SPO11 is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. The human and the mouse SPO11 proteins share 82% amino acid identity, but only 20 to 30% with other eukaryotic homologs such as Drosophila and C. elegans. Both the mouse and human SPO11 exons 2 and 8 are subject to alternative splicing. Northern blot analysis showed that mouse Spo11 is expressed in testis and thymus as a 1.8-kb mRNA, whereas human SPO11 is expressed in testis as a 2.0-kb transcript. RT-PCR revealed that human SPO11 is also expressed in prostate, fetal testis, thymus, and some carcinoma cell lines.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
meiotic recombination protein SPO11 isoform c
NCBI Official Synonym Full Names
SPO11 meiotic protein covalently bound to DSB
NCBI Official Symbol
Spo11
NCBI Official Synonym Symbols
Spo11a; Spo11b; AI449549
NCBI Protein Information
meiotic recombination protein SPO11
UniProt Protein Name
Meiotic recombination protein SPO11
Protein Family
UniProt Gene Name
Spo11
UniProt Entry Name
SPO11_MOUSE
Research Articles on SPO11
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Product Notes
The Mouse SPO11 spo11 (Catalog #AAA9716843) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716843 ELISA Kit recognizes Mouse SPO11. It is sometimes possible for the material contained within the vial of "Meiotic Recombination Protein SPO11 (SPO11), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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