Human Lysosome-associated membrane glycoprotein 2 ELISA Kit | LAMP2 elisa kit

Human Lysosome-associated membrane glycoprotein 2 ELISA Kit

Cat. Num. AAA765274

• Gene Names: LAMP2; LAMPB; CD107b; LAMP-2; LGP110
• Reactivity: Human
• Synonyms: Lysosome-associated membrane glycoprotein 2; Human Lysosome-associated membrane glycoprotein 2 ELISA Kit; LAMP2/Lysosome-associated membrane glycoprotein 2/CD107 antigen-like family member B/LAMPB/LGP110/CD107b/CD107b antigen/LAMP-2/LAMPB; LAMP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LAMP2. No significant cross-reactivity or interference between LAMP2 and analogues was observed.
• Sequence Length: 410

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMP2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504957
• NCBI GeneID: 3920
• NCBI Accession #: NP_002285.1
• NCBI GenBank Nucleotide #: NM_002294.2
• Molecular Weight: 45,170 Da
• NCBI Official Full Name: lysosome-associated membrane glycoprotein 2 isoform A
• NCBI Official Synonym Full Names: lysosomal associated membrane protein 2
• NCBI Official Symbol: LAMP2
• NCBI Official Synonym Symbols: LAMPB; CD107b; LAMP-2; LGP110
• NCBI Protein Information: lysosome-associated membrane glycoprotein 2
• UniProt Protein Name: Lysosome-associated membrane glycoprotein 2
• Protein Family: Lysosome-associated membrane glycoprotein
• UniProt Gene Name: LAMP2
• UniProt Synonym Gene Names: LAMP-2; Lysosome-associated membrane protein 2
• UniProt Entry Name: LAMP2_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

LAMP2: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Defects in LAMP2 are the cause of Danon disease (DAND); also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. Belongs to the LAMP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: extracellular space; integral to membrane; late endosome; late endosome membrane; lysosomal lumen; lysosomal membrane; lysosome; membrane; phagocytic vesicle membrane; plasma membrane; platelet dense granule membrane

Molecular Function: enzyme binding; protein binding; protein domain specific binding

Biological Process: blood coagulation; platelet activation; platelet degranulation; protein import; protein stabilization; regulation of protein stability

Disease: Danon Disease