Rabbit HSP25 Polyclonal Antibody | anti-HspB1; Heat shock protein 25 antibody

HSP25 polyclonal antibody (PE conjugate)

Cat. Num. AAA565301

• Gene Names: Hspb1; 27kDa; Hsp25
• Reactivity: Human, Mouse, Rat, Bovine, Dog, Gerbil, Guinea pig, Hamster, Porcine
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified.
• Synonyms: HSP25; Polyclonal Antibody; HSP25 polyclonal antibody (PE conjugate); anti-HspB1; Heat shock protein 25 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Dog, Gerbil, Guinea pig, Hamster, Porcine
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse, rat, bovine, dog, gerbil, guinea pig, hamster, and pig HSP25.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS containing 0.09% sodium azide.
• Sequence Length: 209

• Applicable Applications for anti-HspB1; Heat shock protein 25 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Flow Cytometry (1:100); Suggested dilutions/conditions may not be available for all applications.
• Immunogen: Recombinant mouse Hsp25.
• Preparation and Storage: Long term: 4 degree C; Avoid vigorous centrifugation and vortexing. Do not freeze. Protect from light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of 106 Jurkat cells stained using HSP25 pAb, PE Conjugate at a concentration of 10µg/ml.)

Related Product Information for anti-HspB1; Heat shock protein 25 antibody

In response to adverse changes in their environment, cells from many organisms increase expression of a class of proteins referred to as heat shock or stress proteins. Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa. Studies on estrogen-induced proteins in breast cancer cells suggest a direct relationship between elevated stress protein expression and neoplasia. Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g., phorbol esters) and calcium ionophores1 and elevated levels of Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock resembles the ecdysteroid and heat shock activation of the drosophila Hsp20 genes. This antibody reacts with both the phosphorylated and the non-phosphorylated forms of Hsp25.

Product Categories/Family for anti-HspB1; Heat shock protein 25 antibody

Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 547679
• NCBI GeneID: 15507
• UniProt Accession #: P14602
• Molecular Weight: 19,380 Da
• NCBI Official Full Name: Heat shock protein beta-1
• NCBI Official Synonym Full Names: heat shock protein 1
• NCBI Official Symbol: Hspb1
• NCBI Official Synonym Symbols: 27kDa; Hsp25
• NCBI Protein Information: heat shock protein beta-1
• UniProt Protein Name: Heat shock protein beta-1
• UniProt Gene Name: Hspb1
• UniProt Synonym Gene Names: Hsp25; Hsp27; HspB1; HSP 25; HSP 27
• UniProt Entry Name: HSPB1_MOUSE

Uniprot Description

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Protein type: Motility/polarity/chemotaxis; Chaperone; Heat shock protein

Cellular Component: axon; contractile fiber; cytoplasm; cytoskeleton; dendrite; extracellular space; focal adhesion; I band; intracellular; M band; nucleus; perikaryon; plasma membrane; proteasome complex; synapse; Z disc

Molecular Function: identical protein binding; protein kinase binding; protein kinase C binding; protein kinase C inhibitor activity; ubiquitin binding

Biological Process: negative regulation of protein kinase activity; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; response to unfolded protein