Human Prostatic binding protein ELISA Kit | PBP elisa kit

Human Prostatic binding protein ELISA Kit

Cat. Num. AAA731158

• Reactivity: Human
• Synonyms: Prostatic binding protein; Human Prostatic binding protein ELISA Kit; PBP elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PBP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PBP elisa kit

Principle of the Assay: PBP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PBP. Standards or samples are then added to the microtiter plate wells and PBP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PBP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PBP are added to each well to "sandwich" the PBP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PBP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PBP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 153955350
• NCBI GeneID: 5393499
• NCBI Accession #: YP_001396115.1
• NCBI GenBank Nucleotide #: NC_009706.1
• Molecular Weight: 74,852 Da
• NCBI Official Full Name: Pbp
• NCBI Official Symbol: pbp
• NCBI Protein Information: predicted beta-lactam-inducible penicillin-binding protein; Pbp
• UniProt Protein Name: Pbp
• Protein Family: Pheromone-binding protein
• UniProt Gene Name: pbp
• UniProt Synonym Gene Names: CKL_2732
• UniProt Entry Name: A5N0U8_CLOK5