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Typical Testing Data/Standard Curve (for reference only)

Human hemoglobin, alpha 2 ELISA Kit | HBA2 elisa kit

Human alpha-subunits of hemoglobin, HB-alpha ELISA Kit

Reactivity
Human
Synonyms
hemoglobin; alpha 2; Human alpha-subunits of hemoglobin; HB-alpha ELISA Kit; Human alpha-subunits of hemoglobin (HB-alpha) ELISA Kit; alpha 2 globin; alpha globin; alpha-2 globin; HBA2 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human HB-alpha. No significant cross-reactivity or interference between human HB-alpha and analogues was observed.
Sequence Length
142
Samples
Serum, plasma, lysate for rbc
Assay Type
Quantitative Competitive
Detection Range
0.17 ng/ml-700 ng/ml
Sensitivity
< 0.17 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HBA2 elisa kit
Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for HB-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated HB-alpha. A competitive inhibition reaction is launched between HB-alpha (Standards or samples) and biotin-conjugated HB-alpha with the pre-coated HB-alpha antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of HB-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
15,191 Da
NCBI Official Full Name
Hemoglobin subunit alpha-2
UniProt Protein Name
Hemoglobin subunit alpha-2
UniProt Gene Name
HBA2
UniProt Entry Name
HBA2_CAPHI

Uniprot Description

Function: Involved in oxygen transport from the lung to the various peripheral tissues

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Product Notes

The Human HBA2 hba2 (Catalog #AAA705099) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA705099 ELISA Kit recognizes Human HBA2. It is sometimes possible for the material contained within the vial of "hemoglobin, alpha 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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