Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Leukocyte cell derived chemotaxin 1 (LECT1) Standard Curve.)
Mouse Leukocyte Cell Derived Chemotaxin 1 (LECT1) ELISA Kit | LECT1 elisa kit
Mouse Leukocyte Cell Derived Chemotaxin 1 (LECT1) ELISA Kit
Gene Names
Cnmd; Chmd; ChM-I; Lect1; Bricd3
Reactivity
Mouse
Synonyms
Leukocyte Cell Derived Chemotaxin 1 (LECT1); Mouse Leukocyte Cell Derived Chemotaxin 1 (LECT1) ELISA Kit; LECT1; RP11-93H24.1; BRICD3; CHM-I; CHM1; BRICHOS domain containing 3; chondromodulin I; LECT1 elisa kit
Reactivity
Mouse
Specificity
Mouse Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LECT1. No significant cross-reactivity or interference between Mouse LECT1 and analogues was observed.
Sequence Length
338
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Leukocyte cell derived chemotaxin 1 (LECT1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Leukocyte cell derived chemotaxin 1 (LECT1) Standard Curve.)
Related Product Information for LECT1 elisa kit
Description: This Mouse Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit employs a two-site sandwich ELISA to quantitate LECT1 in samples. An antibody specific for LECT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LECT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LECT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LECT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: LECT1 is a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. LECT1 expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone.
Background: LECT1 is a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. LECT1 expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
leukocyte cell-derived chemotaxin 1 isoform 1
NCBI Official Synonym Full Names
chondromodulin
NCBI Official Symbol
Cnmd
NCBI Official Synonym Symbols
Chmd; ChM-I; Lect1; Bricd3
NCBI Protein Information
leukocyte cell-derived chemotaxin 1
UniProt Protein Name
Leukocyte cell-derived chemotaxin 1
Protein Family
UniProt Gene Name
Lect1
UniProt Synonym Gene Names
Chmi; CH-SP; ChM-I
Uniprot Description
Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development (). Inhibits in vitro tube formation and mobilization of endothelial cells (). Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization.
Research Articles on LECT1
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Product Notes
The Mouse LECT1 lect1 (Catalog #AAA9717798) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717798 ELISA Kit recognizes Mouse LECT1. It is sometimes possible for the material contained within the vial of "Leukocyte Cell Derived Chemotaxin 1 (LECT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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