Typical Testing Data/Standard Curve (for reference only)
Porcine CytoKeratin 20 ELISA Kit | CK 20 elisa kit
Porcine CytoKeratin 20 ELISA Kit
Reactivity
Porcine
Synonyms
CytoKeratin 20; Porcine CytoKeratin 20 ELISA Kit; CK 20 elisa kit
Reactivity
Porcine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CK 20 elisa kit
Intended Uses: This CK17 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CK17. This ELISA kit for research use only!
Principle of the Assay: CK17 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CK17 antibody and an CK17-HRP conjugate. The assay sample and buffer are incubated together with CK17-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CK17 concentration since CK17 from samples and CK17-HRP conjugate compete for the anti-CK17 antibody binding site. Since the number of sites is limited, as more sites are occupied by CK17 from the sample, fewer sites are left to bind CK17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CK17 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CK17 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CK17 antibody and an CK17-HRP conjugate. The assay sample and buffer are incubated together with CK17-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CK17 concentration since CK17 from samples and CK17-HRP conjugate compete for the anti-CK17 antibody binding site. Since the number of sites is limited, as more sites are occupied by CK17 from the sample, fewer sites are left to bind CK17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CK17 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CK 20 elisa kit
NCBI and Uniprot Product Information
Uniprot Description
K20: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: cytoplasm; intermediate filament
Molecular Function: protein binding; structural constituent of cytoskeleton
Biological Process: regulation of protein secretion; apoptosis; cellular response to stress; intermediate filament organization
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Product Notes
The Porcine CK 20 krt20 (Catalog #AAA739675) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA739675 ELISA Kit recognizes Porcine CK 20. It is sometimes possible for the material contained within the vial of "CytoKeratin 20, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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